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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Virology. 2019 Dec 6;541:52–62. doi: 10.1016/j.virol.2019.11.018

Fig. 5. ZIKV NS5-host cell protein interactions.

Fig. 5.

A. ZIKV NS5 constructs used for yeast two-hybrid screens. ZIKV NS5 gene fragments were fused to the 3’ end of the Gal4 DNA-binding domain. Numbers indicate amino acids.

B. Human genes identified in the yeast two-hybrid screens were cloned into the yeast two-hybrid activation domain vector and retested against all three ZIKV NS5 constructs by assessing yeast growth on SD-TLUH plus 1 mM 3-AT. Interactions were considered positive if at least two out of three independent assays demonstrated growth greater than negative control strains.

C. Plate map of constructs tested for interaction with ZIKV NS5.