Figure 4.

Concentration-response to insulin in isolated gonadal white adipose tissue (gWAT) feed arteries (A) and maximal vasodilation to insulin in the absence or presence of the endothelial nitric oxide synthase inhibitor, L-NAME (B) in normal chow (NC) and high fat (HF) fed wildtype (WT) and roundabout receptor 4 (Robo4) knockout (KO) mice. WT and KO mice were fed either NC or HF (40% fat by kcal) for 8–10 weeks before euthanasia for tissue collection. gWAT feed arteries were excised, cleared of surrounding tissues and cannulated in the stage of a pressure myograph (DMT Inc.) Arteries were preconstricted with phenylephrine and vasodilation was assessed after the cumulative addition of insulin in the absence or presence of L-NAME. *denotes difference from WT-NC, †denotes difference from HF fed of same genotype, Data are means ± SEM, p≤0.05