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. 2020 Feb 17;85(2):147–166. doi: 10.1134/S0006297920020030

Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

O L Bodulev 1, I Yu Sakharov 1,✉
PMCID: PMC7223333  PMID: 32093592

Abstract

Recently, there has been a rapid progress in the development of techniques for isothermal amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The advantage of these methods is that the nucleic acids amplification can be carried out at constant temperature, unlike PCR, which requires cyclic temperature changes. Moreover, isothermal amplification can be conducted directly in living cells. This review describes the principles of isothermal amplification techniques and demonstrates their high efficiency in designing new highly sensitive detection methods of nucleic acids and enzymes involved in their modifications. The data on successful application of isothermal amplification methods for the analysis of cells and biomolecules with the use of DNA/RNA aptamers are presented.

Keywords: nucleic acids, amplification, isothermal, bioanalysis, aptamers

Abbreviations

CHA

catalytic hairpin assembly

EASA

exonu-clease Ill-assisted signal amplification

EXPAR

exponential amplification reaction

HCR

hybridization chain reaction

HDA

helicase-dependent amplification

ICSDP

isothermal circular strand displacement polymerization

LAMP

loop-mediated isothermal amplification

MDA

multiple displacement amplification

NASBA

nucleic acid sequence-based amplification

pWGA

primase-based whole genome amplification

RCA

rolling circle amplification

RPA

recombinase polymerase amplification

SDA

strand-displacement amplification

WGA

whole genome amplification

Funding

This work was supported by the Russian Science Foundation (grant 17-14-01042).

Ethical approval

This article does not contain studies with human participants or animals performed by any of the authors.

Footnotes

Russian Text © The Author(s), 2020, published in Biokhimiya, 2020, Vol. 85, No. 2, pp. 174–196.

Conflict of interest

The authors declare no conflict of interest in financial or any other sphere.

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