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. 2020 Apr 10;10(4):582. doi: 10.3390/biom10040582

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Anti-inflammatory effects of Phormidium EPS after caudal fin amputation induced inflammation in zebrafish larvae. (A) Recovery from inflammation was analyzed with the zebrafish transgenic line NFκB:GFP,Luc. EPS treatment determined a significant reduction of Luciferase activity recorded 48 h after fin amputation. (B) EPS treatment significantly decreased the number of neutrophils in the damaged area of 3 dpf tail fin amputated larvae in LysC:DsRed background. The data represent the mean ± SEM (A) and the mean ± SD (B) of three independent experiments conducted with 12–15 larvae. amp. = amputation. Statistical analysis was performed using GraphPad Prism 7 (one-way ANOVA followed by Tukey’s multiple comparison test). Statistical significance: *** p ≤ 0.001, * p ≤ 0.05.