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. 2020 Apr 23;9(4):1057. doi: 10.3390/cells9041057

Figure 1.

Figure 1

Chlorpromazine (CPZ) causes basolateral redistribution of aquaporin-2 (AQP2) in polarized Madin–Darby canine kidney (MDCK) cells: AQP2-MDCK cells were untreated (left panels) or treated with either vasopressin (VP)/forskolin (FK) (middle panels) or CPZ (CPZ, 100 µM for 15 min) (right panels). After treatment, AQP2 or clathrin were immunodetected. The larger panels represent confocal sections through the middle region of the cell monolayer while the smaller horizontal strips at the bottom of each panel are Z-sections (taken in the plane indicated by the white arrows) that show apical and basolateral membranes. VP/FK treatment resulted in apical redistribution of AQP2 and clathrin, whereas after CPZ treatment, basolateral AQP2 and clathrin signals were clearly increased. The images are representative of three independent experiments. Bar = 10 µm (for all panels).