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. 2020 Mar 26;9(4):806. doi: 10.3390/cells9040806

Figure 3.

Figure 3

Evaluation of GAL1 expression in UC cell lines and verification of downregulation efficiency through Western blotting and q-PCR analyses: Data are presented as mean ± standard error of the mean from three independent experiments. (A,B) High expression of GAL1 in the BFTC-909 and T24 cell lines and low GAL1 expression in the J82 and T24 cell lines were observed. (C,D) The efficiency of GAL1 knockdown by using shRNA in the BFTC-909 and T24 cell lines was assessed through Western blotting and q-PCR analyses. (E,F) The efficiency of GAL1 knockdown by using lentiviruses was assessed through Western blotting and q-PCR analyses in the BFTC-909 cell line. (G,H) The efficiency of GAL1 knockdown by using lentiviruses was assessed through Western blotting and q-PCR analyses in the T24 cell line. * p < 0.05, # p < 0.001.