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. 2020 Apr 23;9(4):1053. doi: 10.3390/cells9041053

Figure 3.

Figure 3

Conditioned medium (CM) modulated SIRT1, increasing histone acetylation and cell invasion of normal kidney cell line. Characterization of SIRT1 expression in normal kidney cell line treated with conditioned medium from tumor cells, by RT-qPCR (A), Western blot (B), and immunofluorescence (C). Characterization of global histone acetylation of histone H3 and specific histone marker H3K9 (D) in normal kidney cell line treated with conditioned medium from tumor cells, by Western blot. Effect of conditioned medium from tumor cells in normal kidney cell line at cell proliferation (BrdU assay) (E), cell migration (wound-healing assay) (F), and cell invasion (Matrigel Invasion Chambers) (G). Western blot and immunofluorescence quantification are represented as fold change of 20 mM lactate versus control condition; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and ns p > 0.05 (non-significant). Abbreviations: CM—conditioned medium, CTR—control.