Table 2.
Antibodies used for immunofluorescence (IF) and Western blotting (WB).
| Antibody | Antigen | Species and Specifications | Concentration | Reference | Source |
|---|---|---|---|---|---|
| Ab1 | SLC5A3 | rabbit anti-AA221-270 | IF 2.5 µg/mL | B4872/27459 | LS-Bio |
| Ab2 | SLC5A3 | rabbit anti-central region | IF 10 µg/mL | C358323/125465 | LS-Bio |
| Ab3 | SLC5A3 | mouse anti-AA533-642 IgG1 | WB 0.8 µg/mL | SAB1402920 | Sigma |
| Ab4 | GAPDH | mouse IgG1 | WB 0.2 µg/mL | B9310/127452 | LS-Bio |
| Ab5 | SLC5A11 | rabbit polyclonal IgG | IF 4 µg/mL | HPA035331 | AtlasAntibodies |
| Ab6 | CD3 | mouse IgG1 | IF 10 µg/mL | M7254 | Dako |
| Ab7 | CD56 | mouse IgG1 | IF 5 µg/mL | MAB2120Z | Millipore |
| Ab8 | CD68 | mouse IgG1 | IF 10 µg/mL | Ab955 | Abcam |
| Ab9 | CD206 | mouse IgG1 | IF 0.4 µg/mL | MCA2155 | Biorad |
| Ab10 | SQSTM1 | mouse anti-AA257-437 IgG1 | IF 1.3 µg/mL | 610833 | BDTransduction |
Na+/myo-inositol cotransporter 1/solute carrier A3 (SLC5A3); glyceraldehyde-3-phosphate dehydrogenase (GAPDH); sequestosome 1/p62 (SQSTM1).