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Elevated levels of interleukin (IL)-6 and IL-8 secreted into the culture medium of Tat- or Nef-treated ASCs. After 15 days of treatment with Tat or Nef, the levels of (A) IL-6 and (B) IL-8 in the culture medium from the last 24 h were determined with ELISAs (n = 3, in duplicate). The results are expressed as the mean ± SEM. Experiments were performed in duplicate on ASCs isolated from different abdominal SCAT donors. * P < 0.05 vs. control cells.
