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Wnt role in pluripotency and lineage specification during bovine BL development. (a,b) Representative images of pluripotency and lineage specific markers in control and 6-Bio (400 nM) treated group. DAPI labeled nuclei in blue. (c) Bar graph data of mean fluorescence intensities presented as a means ± SEM from three independent sets of experiment, including n = 20 BLs per group in each replicate. ** p < 0.01; *** p < 0.001 indicates significant difference. Original magnification 100×.
