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. 2020 Apr 9;9(4):923. doi: 10.3390/cells9040923

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Wnt stimulate fatty acid oxidation (FAO) metabolism through PPARδ-Cpt1-axis to regulate BL development and Hatching. (a,b) qRT-PCR analysis for FAO regulating genes Cpt1 and Pdk4 expression by 6-Bio (400 nM), Gsk3787 (15 µM) and 6-Bio + Gsk3787 treated groups. n = 5 BLs. (c) Immunofluorescence imaging showing Cpt1 expression. DAPI labeled nuclei in blue. (d) Bar graph data of mean fluorescence intensities presented as a means ± SEM from three individual sets of experiment, including n = 20 BLs per group in each replicate. * p < 0.05; ** p < 0.01; *** p < 0.001 indicates significant difference. Original magnification 100×.

Wnt stimulate fatty acid oxidation (FAO) metabolism through PPARδ-Cpt1-axis to regulate BL development and Hatching. (a,b) qRT-PCR analysis for FAO regulating genes Cpt1 and Pdk4 expression by 6-Bio (400 nM), Gsk3787 (15 µM) and 6-Bio + Gsk3787 treated groups. n = 5 BLs. (c) Immunofluorescence imaging showing Cpt1 expression. DAPI labeled nuclei in blue. (d) Bar graph data of mean fluorescence intensities presented as a means ± SEM from three individual sets of experiment, including n = 20 BLs per group in each replicate. * p < 0.05; ** p < 0.01; *** p < 0.001 indicates significant difference. Original magnification 100×.