Table 2.
Co-treatment with 6-Bio rescued the inhibitory effect of PPARδ antagonist (Gsk3787) on embryonic development to the BL stage and hatching.
| Groups | Oocytes, n | Speculated Zygotes, n | Cleaved Embryos, % | Total Blastocyst, % | Hatched Blastocyst, % |
|---|---|---|---|---|---|
| Control | 502 | 481 | 377 (78.5 ± 0.2) c | 140 (29.2 ± 0.5 ) c | 63 (45.0 ± 0.9) c |
| 6-Bio (400 nM) | 521 | 496 | 387 (78.2 ± 0.3) c | 188 (37.8 ± 1.3) d | 115 (61.1 ± 1.6) d |
| Gsk3787 (15 µM) | 453 | 430 | 323 (75.0 ± 0.4) a | 69 (16.1 ± 0.6) a | 5 (7.2 ± 2.5) a |
| 6-Bio + Gsk3787 | 521 | 494 | 378 (76.7 ± 0.4) b | 120 (24.3 ± 0.4 ) b | 43 (35.5 ± 1.2) b |
a,bp < 0.05 with different superscript in the column indicate significant difference.