Figure 3.

HPLC analysis at t=0, 3 h and 16 h of the transglycosylation reaction of 5 in the presence of thymidine (4 equiv) and NDT (0.63 μL/μmol acceptor) at 37 °C. HPLC conditions: 0–20 % linear gradient of acetonitrile in 10 mm TEAA buffer (pH 6.0) over 20 min at a flow rate of 1 mL min⁻¹. Detection at 254 nm.