The effect of red clover extract (RC) and red clover anthocyanins fraction (RCA) on gene expression and TNFα secretion in LPS-stimulated RAW 264.7 macrophages. Expression of genes related to pro-inflammatory markers was determined in LPS-stimulated RAW 264.7 cells that were treated with or without RC (A–E) or RCA (F–J) at concentrations of 5, 10, or 20 μg/mL. (K) Concentrations of TNFα that was released in the media were measured. A different letter indicates a statistically significant difference (P < 0.05). + and - indicate the presence and absence of LPS, RC, or RCA, respectively.