Figure 2.
The effect of RCA on intracellular ROS production and the expression of gene and proteins related to oxidative stress. (A) Intracellular ROS levels were determined in LPS-stimulated RAW 264.7 cells that were treated with or without 5 or 10 μg/mL RC or RCA. NOX1 (B) and NRF2 (D) genes were measured using qPCR. (C) p47phox and its phosphorylation were determined using a Western blot. A different letter indicates a statistically significant difference (P < 0.05). + and - indicate the presence and absence of LPS, RC, or RCA, respectively.