Table 1.
The sequences of forward and reverse primers for target genes.
| Gene | Direction | Sequence (5’-3’) |
|---|---|---|
| MuRF1 | Forward | TCTGGACTTAGAACACATAGCAGAG |
| Reverse | TCTCCTTCTTCATTGGTGTTCTTCT | |
| Atrogin-1 | Forward | CAGTGATCCATTCTGTTCATCCTTG |
| Reverse | TTATTTCCAGCCAAATGGAGAGAGA | |
| Beclin1 | Forward | CTCCTTAGGGGATGTTTGCCTT |
| Reverse | TCAGGAAAGAGGGAAAGGATGC | |
| LC3 | Forward | GGGAGGTCCTGGCTCCTAAA |
| Reverse | CAGACAGGCAAGGGCCTAAC | |
| Atg4 | Forward | GAGCCTTCCTCCATGTTCTTTCTC |
| Reverse | CTCATATCTAGGGGGAGGAAAGGT | |
| Atg7 | Forward | TAGAGAGGACCTTGGCCTAACA |
| Reverse | CAAGCCATCTATGTGTGTGCTG | |
| PGC-1α | Forward | GAGTGTTCTGGTACCCAAGGC |
| Reverse | CTGGGCCGTTTAGTCTTCCTT | |
| NRF-1 | Forward | AGATTCGTGGGTGGTAGGGT |
| Reverse | TCTAGCAGAGGTCTAGGCGG | |
| Tfam | Forward | AGACTACACTGGGAAACCACAG |
| Reverse | GGCTTATAGGGACCCAGTGATG | |
| IL-6 | Forward | AGACAAAGCGAGAGTCCTTCAG |
| Reverse | GTCCTTAGCCACTCCTTCTGTG | |
| TNF-α | Forward | GAGTCATTGCTCTGTGAAGGGA |
| Reverse | ATTCTGAGACAGAGGCAACCTG | |
| Genomic DNA | Forward | GCCAGCCTCTCCTGATTTTAGTGT |
| Reverse | GGGAACACAAAAGACCTCTTCTGG | |
| Mitochondria DNA | Forward | CCGCAAGGGAAAGATGAAAGAC |
| Reverse | TCGTTTGGTTTCGGGGTTTC | |
| β-Actin | Forward | GAAGGAGATTACTGCTCTGGCTC |
| Reverse | CTCAGTAACAGTCCGCCTAGAA |