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. 2020 Apr 10;12(4):429. doi: 10.3390/v12040429

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EVs-transferred BoHV-1 proteins or specifically gB can bind virus-specific antibodies from the whole virus-specific animal serum. (A) EVs gB ELISA was performed with the goat anti-BoHV-1 serum.. The signal from gB-containing EVs was compared with the signal from MJS/MDBKpuro. (B) and (C) ELISA detection of BoHV-1 proteins in OptiPrep-isolated material (fractions vF11, vF15 and vF21) from BoHV-1-infected MDBK (B) or MJS (C) cells. The signal from virus-infected material was compared with the signal from the corresponding fractions F11 or F21 from uninfected MJS/MDBK (designated as Ø). A total of 1% BSA in PBS served as a background control. Each reaction was performed in triplicate and bars represent means of absorbance at 450 nm with the standard deviations; * p ≤ 0.01, ** p ≤ 0.001.