Table 1.
E. coli strains and plasmids.
| Strain or Plasmid | Description | Source or Reference |
|---|---|---|
| 43895 | E. coli O157:H7 ATCC 43895, a clinical isolate, stx1+/stx2+, curli+ | ATCC * |
| manC-Tn5 | Tn5 inserted in manC of 43895 | This work |
| per-Tn5 | Tn5 inserted in per of 43895 | This work |
| csgB-Tn5 | Tn5 inserted in csgB of 43895 | This work |
| waaI-Tn5 | Tn5 inserted into waaI of 43895 | This work |
| wzy-Tn5 | Tn5 inserted into wzy of 43895 | This work |
| 43895ΔcsgB | 43895 with csgB deletion | This work |
| 43895ΔcsgB(pcsgBA) | 43895ΔcsgB complemented csgB | This work |
| 43894 | E. coli O157:H7 ATCC 43894, a clinical isolate, stx1+/stx2+, curli- | ATCC |
| 43894R | 43894 mutant with A to T transversion in csgD promoter, curli+ | This work |
| Sakai SakaiR |
E. coli O157:H7, a clinical isolate, stx1+/stx2+, curli- Sakai mutant with A to T transversion in csgD promoter, curli+ |
[16] This work |
| FB38 | E. coli O157:H7, a bovine isolate, stx1+/stx2-, curli positive | Laboratory stock |
| S17-1 λ pir | pro recA thi hsdR Hfr RP4-2 (Tc::Mu) (Km::Tn7, SmR, λ pir lysogen | [38] |
| K-12 | E. coli MG1655 strain | Laboratory stock |
| pKD4 | Template plasmid for mutagenesis (AmpR KanR) | [37] |
| pKD3 | Template plasmid for mutagenesis (AmpR CmR) | [37] |
| pKD46 | Red recombinase helper plasmid, RepA101(Ts), AmpR | [37] |
| pACYC177 | Cloning vector, AmpR, KanR | ATCC |
| pGP704.L | Suicide plasmid, mob+, sacBR+, pir-dependent oriR6K, AmpR | [39] |
| pGP704csg1 | pGP704.L containing csgBA genes of 43895; allelic exchange plasmid | This work |
| pcsgBA | csgBA and intergenic region of 43895 cloned into pACYC177 | This work |
* ATCC, American Type Culture Collection, Manassas, VA.