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. 2020 Apr 1;12(4):392. doi: 10.3390/v12040392

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Suppression of nbe-miR1919c-5p expression using a TRV-based vector enhances leaf curling symptoms caused by TbCSV/TbCSB infection. (A) Plants inoculated with TRV or TRV-M1919 were photographed at 7 dpi. The TRV-M1919-inoculated plant showed stronger leaf curling symptoms compared with the TRV-inoculated control plants (TRV-CK). (B) Results of qRT-PCR showed that the expression of nbe-miR1919c-5p in the TRV-M1919-inoculated plants was significantly reduced compared with the control plants. (C) The TRV+TbCSV/TbCSB-inoculated and the TRV-M1919+TbCSV/TbCSB-inoculated plants were photographed 7 days after the second inoculation. The TRV-M1919+TbCSV/TbCSB-inoculated plants showed severe leaf curling symptoms. (D) Results of qRT-PCR showed that the expression of nbe-miR1919c-5p in the TRV-M1919+TbCSV/TbCSB-inoculated plants was significantly reduced compared with that in the TRV+TbCSV/TbCSB-inoculated plants. (E) Detection of TbCSV DNA copy number in the inoculated and the systemic leaves harvested from the TRV+TbCSV/TbCSB-inoculated or the TRV-M1919+TbCSV/TbCSB-inoculated plants. (F) Detection of TbCSB DNA copy number in the inoculated and the systemic leaves harvested from the TRV+TbCSV/TbCSB-inoculated or the TRV-M1919+TbCSV/TbCSB-inoculated plants. * p < 0.05 by the Student’s t-test.