Table 3.
Diversity, frequency, and molecular features of Giardia duodenalis sequences at the gdh, bg and tpi loci obtained in the children population under study in Leganés, Madrid, 2017–2019. GenBank accession numbers are provided. Point mutations inducing amino acid substitutions are highlighted as superscript letters indicating the amino acid change.
| Locus | Assemblage | Sub-Assemblage | Isolates | Reference Sequence | Stretch | Single Nucleotide Polymorphisms | GenBank ID |
|---|---|---|---|---|---|---|---|
| gdh | A | AII | 3 | L40510 | 64–496 | None | MN844134 |
| 1 | 76–491 | T269Y1 | MN844135 | ||||
| B | BIV | 6 | L40508 | 76–491 | None | MN844136 | |
| 1 | 76–496 | C102Y, C105Y, C432Y, C435Y | MN844137 | ||||
| 1 | 78–485 | C126Y | MN844138 | ||||
| 5 | 76–441 | T183C, T387C, C396T, C423T | MN844139 | ||||
| 2 | 76–482 | T183Y, T387Y, C396Y, C423Y | MN844140 | ||||
| 1 | 76–496 | T183Y, T387Y, C423Y | MN844141 | ||||
| BIII/BIV | 1 | 76–496 | C85Y2, T135Y, T183Y, G186R, C255Y, C273Y, C345Y, T366Y, C372Y, T387Y, A438R, G453R | MN844142 | |||
| bg | A | AII | 3 | AY072723 | 96–604 | None | MN844143 |
| B | B | 3 | AY072727 | 93–593 | G159A, C165T, C309T, C324T, C393T, T471C | MN844144 | |
| 1 | 102–590 | G159A, C165T, A265R3, C309T, C324T, C393T, T471C | MN844145 | ||||
| 1 | 93–604 | G159A, C165T, C309T, C324T, C352T4, C393T, T471C | – | ||||
| 1 | 102–604 | G159A, C165T, C309T, C324T, C372T, C393T, T471C | MN844146 | ||||
| tpi | A | AII | 2 | U57897 | 282–751 | None | MN844147 |
| 1 | 276–805 | C287G, A291W5 | MN844148 | ||||
| B | BIV | 7 | AF069560 | 1–479 | None | MN844149 | |
| 1 | 1–479 | A201R6 | MN844150 | ||||
| 1 | 1–479 | G305A, G425A, G426A7 | MN844151 |
1 If C, pM90T; 2 If T, pP29S; 3 If G, pK89E; 4 Stop codon; 5 If T, pN95Y; 6 If G, pT74A; 7 pE143K; R: A/G; W: A/T; Y: C/T.