Skip to main content
. 2020 Apr 21;8(4):603. doi: 10.3390/microorganisms8040603

Figure 5.

Figure 5

Effects of DON, NX-3, AURO and their combinations on the cellular protein content (A,B) and viability (C,D) of the two human colon cell lines HT-29 and HCEC-1CT determined in the sulforhodamine B (HT-29: A; HCEC-1CT: B) and alamarBlue® assay (HT-29: C; HCEC-1CT: D). Cells were preincubated with the compounds (DON, NX-3, AURO and their combinations) for 2 h followed by a 3 h IL-1β challenge (25 ng/mL). Data are expressed as mean values ± SD normalized to IL-1β-treated solvent control samples of at least five independent experiments. One percent DMSO and 1% water (LC–MS grade) served as solvent control (dotted line). Significant differences to IL-1β-treated solvent control, calculated with one-sample t-test, are indicated with * (p < 0.05), ** (p < 0.01) and *** (p < 0.001), whereas differences between DON, NX-3 and their combinations with AURO, calculated with a two-sample t-test, are indicated with ° (p < 0.05), °° (p < 0.01) and °°° (p < 0.001).