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. 2020 Mar 28;12(4):373. doi: 10.3390/v12040373

Figure 5.

Figure 5

Menthol treatment reduces mitochondrial fission basally and during CVB infection- HeLa cells were treated with 1 mM menthol and then examined after 6 h. (A) Western blot on cell lysates detecting mitochondrial fission marker phospho-DRP1Ser616 (pDRP1). Ponceau S is shown below. (B) Densitometric quantification of western blot in A. HeLa cells were treated with 1 mM menthol or equivalent dose of ethanol for 1 h and subsequently infected with EGFP-CVB at MOI 0.1 for 24 h. (C) Western blot on cell lysates detecting pDRP1. Ponceau S is shown below. (D) Densitometric quantification of western blot in C. (* p < 0.05, *** p < 0.001; Student’s t-test, n = 3–4).