Figure 4.

T Cells Migrate on Perivascular Stromal Cells Connecting the Red Pulp to T Zones

(A and B) Fixed spleens from hCD2-DsRed mice were sliced with a vibratome (150 μm thick) and stained for PDGFR-β (A) or ER-TR7 (B). Micrographs show high magnifications of areas highlighted in dotted boxes. Sections were imaged by TPLSM.

(C) Left, snapshot from intravital imaging of spleens from a hUbi-GFP (green, stroma) recipient chimera reconstituted with hCD2-DsRed bone marrow cells (red, T cells). Right, zoom on two boxed regions showing a single z-plane.

(D) Sections from Ccl19-iEYFP.2 chimeras reconstituted with hCD2-DsRed bone marrow stained with anti-GFP (green). Two single z-planes from the indicated boxed region are shown on the right with additional micrographs for further magnifications of marked areas (dotted box).

(E) Fixed spleens from hCD2-DsRed mice were vibratome-sliced (150 μm thick) and stained for CCL21 (green). Two single z-planes from boxed region are shown on the right with additional micrographs for further magnifications of marked areas (dotted box). Data are representative of two mice.

(F) Fixed sections from Ccl19-iEYFP.2 chimeras reconstituted as in (D) were stained with anti-GFP (green) and podoplanin (white). Sections were imaged by confocal microscopy.

Data in (D) and (F) represent three mice, with at least six sections per mouse.