Figure 4.

Representative ¹H-¹³C HSQC spectra of cell extracts after 24 h of culture with 10 mmol/L 1-¹³C-glucose and 3 mmol/L 1-¹³C acetate (A) or 1-¹³C-galactose and 3 mmol/L 1-¹³C acetate (B). (C,D) correspond to the ¹H-¹³C HMBC spectra of the samples shown in (A,B) respectively. The ratio between glutamate C4 and lactate C3 (E) is indicative of the pathway of glucose oxidation while glutamate C5 (F) is indicative of β-oxidation. n = 3 for double label experiments, * statistically different p < 0.05.