Background. To determine the frequency of detecting potentially pathogenic bacterial and viral gene targets in respiratory specimens collected from symptomatic patients during the 2013-2014 influenza season.
Methods. A retrospective study involving 264 hospitalized patients with respiratory symptoms. Respiratory samples were obtained and screened for 22 different viruses, 16 bacteria, and 5 genetic drug resistance targets using a Target Enriched Multiplex Polymerase Chain Reaction.
Results. We enrolled 264 hospitalized patients. Age range of 1-100 years with mean age of 57 years, 48.5 % male and 51.5 % females, 25% had both viral and bacterial gene targets detected while 17% had more than one bacterial target detected and 9% had more than one viral target detected. Positive viral targets were found in 57.2% subjects, of these: 54% were H1N1, 24.5% RSV, 11.2% rhinovirus, 15.9% coronavirus, and 9.3% Coxsackie virus. Positive bacterial targets were found in 65%, majority of which were Staphylococcus aureus (60%), Moraxella (11%), Pseudomonas (20%), Streptococcus Pneumoniae (19%) and Hemophilus Influenzae (14%). MRSA accounted for 81.5 % of S. aureus and 12 % of those were PVL gene positive. Positive H1N1 samples were found in 30.68% subjects. Of all the H1N1 positive subjects; 59 % were females, 23 % were 51-60, 22% were 61-70 and 16% were 31-40 years. Of all the MRSA positive, 58 % were females, 32% were 61-70. 17% were 51-60, 14% were 71-80 and 11% were 81-90 years. Of all the H1N1 positive patients 25 % had co detection of MRSA, 9 % Pseudomonas and 11% Streptococcus Pneumoniae. Out of the co positive bacterial isolates 17% had co detection of MRSA and Pseudomonas. Out of the co positive viral isolates 16% had co detection of H1N1 and Rhinovirus
Conclusion. Hospitalized patients with respiratory symptoms admitted during the influenza season 2013-2014 showed high rate of H1N1 detected of 30%, it showed co-detection of viral and bacterial targets, female were more commonly infected . Those with other viral infection were commonly RSV and coronavirus. Detection of MRSA target was high in our patient population whether been carrier or infected. H1N1 and MRSA were detected more common in elderly.
Disclosures. All authors: No reported disclosures.