Fig. 2.

In vivo optical imaging of OV-90^luc+xenograft models. (a) Comparison of longitudinal BLI and FLI imaging in orthotopic OV-90^luc+xenograft models (n = 3). CD24-AF680 fluorescence imaging, 24 h after probe injection, visualise the primary tumour and liver metastasis. (b) Ex vivo bioluminescence and fluorescence images demonstrate the tumour manifestation of one representative mouse. (c) Correlation of fluorescence to bioluminescence signal intensity measured in three individual mice over time (r² = 0•14). (d) H&E stained primary tumour tissue and abdominal metastasis and CD24 immunohistochemical staining of OV-90^luc+tumour tissues (staining index = 4, scale: negative 0 – 9 high) and murine ovarian tissue (negative); Scale bars: original magnification of x400 mm. All optical images are presented with the minimum to maximum fluorescence/bioluminescence intensity for each mouse and ex vivo organs. FLI = fluorescence imaging; BLI = bioluminescence imaging; NC = number of counts; IHC = Immunohistochemistry.