Figure 2.

FRET construct and data for toggling of U2 snRNA. (A) The stem II of the U2 snRNA can switch between two conformations. Labeling positions in the two studies of Cy3 (green) and Cy5 (red) as well as Pseudouridylation sites are indicated. (B) Example FRET trajectory of U2 shows a spontaneous toggling between two FRET states. (C) FRET histograms of WT RNA in dependence of Mg²⁺ and Cus2 concentrations. This Figure was adapted and modified from Rodgers et al. [16] and used under Creative Commons Non-Commercial License (CC-BY NC).