Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | C57BL/6J | Jackson Laboratory | RRID:IMSR_JAX:000664 | |
| Genetic reagent (M. musculus) | B6.129S5-Skap2Gt(VICTR20)21Lex/Mmjax | Jackson Laboratory | RRID:MGI:4353994 | |
| Genetic reagent (M. musculus) | B6.129S-Cybbtm1Din/J | Jackson Laboratory | RRID:IMSR_JAX:002365 | |
| Genetic reagent (M. musculus) | BALB/c BALB/cAnNTac |
Taconic Laboratory | RRID:IMSR_TAC:balb | |
| Genetic reagent (Klebsiella pneumoniae) | ATCC 43816 KPPR1 | GenBank ATCC |
GenBank: CP009208.1 | |
| Antibody | Rat Anti-Ly6G monoclonal antibody, unconjugated, Clone 1A8 | Fisher Scientific | BD Biosciences Cat# 551459, RRID:AB_394206 | (100 ul of 50 ug/ml) |
| Antibody | Anti-CCR2 (MC21) | Dr. Matthias Mack Mack et al., 2001 | Cat# CCR2 (MC21), RRID:AB_2314128 | (100 ul of 200 ug/ml) |
| Antibody | Rat anti-mouse CD16/CD32 Mouse BD Fc Block | BD Biosciences | BD Biosciences Cat# 553142, RRID:AB_394657 | (1:200 dilution) |
| Antibody | Rat monoclonal anti-mouse/human α-CD11b-PE or α-CD11b-PacBlue (clone M1/70) |
Biolegend | BioLegend Cat# 101207, RRID:AB_312790
BioLegend Cat# 101223, RRID:AB_755985 |
(1:300 dilution) |
| Antibody | Rat monoclonal anti-mouse α-Ly6G PE-Cy7 (clone 1A8) |
Biolegend | BioLegend Cat# 127617, RRID:AB_1877262 | (1:300 dilution) |
| Antibody | Hamster monoclonal anti-mouse α-CD11c-PerCP-Cy5.5 (clone HL3) | BD Biosciences | BD Biosciences Cat# 560584, RRID:AB_1727422 | (1:300 dilution) |
| Antibody | Rat monoclonal anti-mouse α-Gr1-FITC or α-Gr1-APC (clone RB6-8C5) |
BioLegend | BioLegend Cat# 108411, RRID:AB_313376
BioLegend Cat# 108405, RRID:AB_313370 |
(1:300 dilution) |
| Antibody | Rat monoclonal anti-mouse α-Ly6C-AlexaFluor647 (clone HK1.4) |
BioLegend | BioLegend Cat# 128010, RRID:AB_1236550 |
(1:300 dilution) |
| Antibody | Rabbit polyclonal anti-mouse/human α-SKAP2 | Proteintech | Proteintech Cat# 12926–1-AP, RRID:AB_2189317 | (1:200 dilution or 1.57 ug/ml) |
| Antibody | rabbit IgG polyclonal isotype antibody | Proteintech | Proteintech Cat# 30000–0-AP, RRID:AB_2819035 | (1.57 ug/ml) |
| Antibody | Alexa Fluor 488 goat anti-rabbit secondary antibody | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A-11034, RRID:AB_2576217 | (1:250 dilution) |
| Antibody | Rabbit polyclonal anti-human serum albumin | Sigma-Aldrich | Sigma-Aldrich Cat# A0433, RRID:AB_257887 |
(1:400 dilution) |
| Antibody | Rabbit anti-human/mouse Phospho-Src Family (Y416) | Cell Signaling Technology | Cell Signaling Technology Cat# 2101, RRID:AB_331697 | (1:500) |
| Antibody | Rabbit anti-human/mouse monoclonal Phospho-Zap-70 (Y319)/Syk (Y352) | Cell Signaling Technology | Cell Signaling Technology Cat# 2717, RRID:AB_2218658 | (1:500) |
| Antibody | Rabbit anti-human/mouse polyclonal Phospho-Pyk2 (Y402) | Cell Signaling Technology | Cell Signaling Technology Cat# 3291, RRID:AB_2300530 | (1:500) |
| Antibody | Rabbit anti-human/mouse polyclonal RhoGDI | Cell Signaling Technology | Cell Signaling Technology Cat# 2564, RRID:AB_2274313 | (1:1000) |
| Antibody | Rabbit anti-human/mouse polyclonal Syk | Cell Signaling Technology | Cell Signaling Technology Cat# 2712, RRID:AB_2197223 | (1:500) |
| Antibody | Rabbit anti-human/mouse polyclonal Pyk2 | Cell Signaling Technology | Cell Signaling Technology Cat# 3292, RRID:AB_2174097 | (1:500) |
| Antibody | Goat Anti-rabbit IgG (H+L) (DyLight 800 4X PEG Conjugate) | Cell Signaling Technology | Cell Signaling Technology Cat# 5151, RRID:AB_10697505 | (1:20,000) |
| Recombinant DNA Reagent | MSCVneo-HA-ER-Hoxb8 | Wang et al., 2006 | In-house (Sykes lab @ MGH) | |
| Peptide, recombinant protein | Stem cell factors | Cho-SCF | In-house | |
| Peptide, recombinant protein | Recombinant murine Stem cell factors | Peprotech | Cat. #: AF-250 | (50 ng/ml) |
| Peptide, recombinant protein | Recombinant murine Interleukin-3 | Peprotech | Cat. #: 213–13 | (10–50 ng/ml) |
| Peptide, recombinant protein | Recombinant murine Interleukin-6 | Peprotech | Cat. #: 216–16 | (10 ng/ml) |
| Peptide, recombinant protein | Recombinant human G-CSF | Peprotech | Cat. #: 300–23 | (50 ng/ml) |
| Peptide, recombinant protein | Fibronectin human plasma | Sigma | Cat. #: F0895 | (10 ug/ml) |
| Peptide, recombinant protein | β-estradiol | Sigma | Cat. #: E2758 | (0.5 uM) |
| Peptide, recombinant protein | Firbonectin-like protein polymer genetically engineered | Sigma Aldrich | Cat. #: F5022 | (15 ug/ml) |
| Peptide, recombinant protein | Albumin from human serum | Sigma | Cat. #: A9511 | (20 ug/ml) |
| Chemical compound | Hexadimethrine bromide (polybrene) | Sigma Aldrich | Cat. #: 107689 | (8 ug/ml) |
| Commercial assay or kit | Mouse total MMP9 DuoSet ELISA | R and D Systems | Cat. #: DY6718 | |
| Commercial assay or kit | Mouse neutrophil elastase/ELA2 DuoSet ELISA | R and D Systems | Cat. #: DY4517 | |
| Chemical compound | 4-Aminophthalhydrazide (isoluminol) | Sigma | Cat. #: A8264 | (50 uM) |
| Chemical compound | Peroxidase from horseradish | Sigma | Cat. #: P6782 | (15 U/ml) |
| Chemical compound, drug | PP2 | Selleck | Cat. #: S7008 | (10 nM) |
| Chemical compound, drug | R406 | Selleck | Cat. #: S2194 | (2 uM) |
| Chemical compound, drug | Ibrutinib (PCI-32765) | Selleck | Cat. #: S2680 | (1 uM) |
| Chemical compound, drug | Gouml 6983, PKC inhibitor | Abcam | Cat. #: ab144414 | (10 uM) |
| Chemical compound, drug | U-73122 | Sigma | Cat. #: U6756 | (1 uM) |
| Chemical compound, drug | U-73433 | Sigma | Cat. #: U6756 | (1 uM) |
| Chemical compound, drug | G418/Geneticin | Thermo Scientific | Cat. #: 10131035 | (1 mg/ml) |
| Other | Immunolon 4HBX 96-well plates | Fisher Scientific | Cat. #: 3855 | |
| Other | Cytochrome c from equine heart | Sigma | Cat. #: C7752 | (100 uM) |
| Other | Ficoll-Paque-Plus | Pharmacia/GE Healthcare | Cat. #: GE17-1440-02 | (1:1 dilution) |
| Other | Percoll | Sigma-Aldrich | Cat. #: P1644 | |
| Other | e-Myco Mycoplasma PCR Detection Kit | Bulldog Bio | Cat. #: 25233 |