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. Author manuscript; available in PMC: 2020 May 27.
Published in final edited form as: Oncogene. 2019 Jul 15;38(36):6323–6337. doi: 10.1038/s41388-019-0881-8

Figure 2:

Figure 2:

Rgnef knockout prevents spontaneous ovarian tumor formation. (a) Image of a TAg-stained (brown) ovary and oviduct from a MISIIR-TAg+ mouse. TAg is expressed in the murine ovary and fallopian tubal lesions (green asterisks and dotted lines) and oviduct (yellow asterisk and dotted line). Scale is 1 mm (left) or 100 μM (right). (b) Breeding schematic for generation of Rgnef−/− or Rgnef+/+; MISIIR;TAg+ mice. (c) Ovary size of littermates measured by ultrasound. Mice were sacrificed at week 17 or at earlier humane endpoint. Each point represents n ≥ 5 mice with error bars as SEM. (d) Representative pictures of oviducts and ovaries for each group at endpoint. Scale is 1 cm. (e) Mean tumor burden (total ovary mass) was higher in Rgnef+/+ than Rgnef−/− mice (*P < 0.05, SD +/− 0.93 for Rgnef+/+ and 0.39 for Rgnef−/− mice). (f) Immunofluorescent staining for TAg (green) and DNA (Hoechst, blue) in frozen section of ovaries from Rgnef−/− or Rgnef+/+; MISIIR;TAg+ tumor-bearing mice. Scale is 100 μm.