Fig 6.

Neutralizing of EBV infection. (A) Neutralizing curve for mAb 72A1 against EBV-GFP infection of AKATA cells. M Ab 72A1 was used to validate the neutralization assay and its IC₅₀ is indicated. (B) Neutralization of infection by immune sera. Serially diluted sera collected at week 8 were used to block EBV infection of AKATA cells. Significance (*p≤0.05, **p≤0.01, ***p≤0.001) is indicated for 149-3Aand 149-3B VLPs compared to gp350ECD123. Both constructs 149-3A and 149-3B have the same level of significance above gp350ECD123. (C) Sera collected at week 10 was diluted 10 fold to block EBV infection into AKATA cells (n=5). Significance (*p≤0.05, **p≤0.01, ***p≤0.001) between the indicated fusion proteins versus gp350ECD123 is shown. EBV-GFP show the level in the absence of inhibitors. Neg shows the background level in the absence of infection. 72A1 shows the level of inhibition by mAb 72A1 at 50 μg/ml.