Figure 4.

PRESA eradicated resistant E. coli in a mouse skin infection model. (A) The proportion of MG1655 cells containing pUCtarget_k in infected skin was determined 12 h after treatment with vB_Cas9 or WT temperate phage. Each value represents the percentage of plasmid-positive cells in each isolated mouse skin sample (n = 4, mean ± SD). (B) Infected mouse skin was treated with PRESA, lytic phage vB_253, kanamycin, vB_Cas9, or a PBS control. Data represent the logarithm value of bacterial cell counts per gram of skin (n = 3, mean ± SD).