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. 2020 Apr 30;9:e54441. doi: 10.7554/eLife.54441

Figure 9. Simulations: Segregated expression reduces buffering effects.

Figure 9.

(A) Schematics of simulation in which EAATs and iGluSnFRs were colocalized (i), or segregated into alternating spherical shells surrounding the synapse, with EAATs occupying the innermost shell (ii). (B) Concentration profile of EAATs and iGluSnFRs in the 2 μm shell case. In each case, the average EAAT and iGluSnFR concentrations were 100 μM and 300 μM, respectively. (C) Simulated iGluSnFR signal wave forms at four different shell thicknesses. (D) Glutamate clearance time courses in the same simulations described in C. (E) Simulated synaptic transporter currents (STCs) in the 2 μm shell condition and in the absence of iGluSnFR. (F-J) As in Aii-E, except that EAATs were distributed evenly throughout all shells.