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. Author manuscript; available in PMC: 2021 Feb 6.
Published in final edited form as: Cell. 2020 Jan 23;180(3):502–520.e19. doi: 10.1016/j.cell.2019.12.024

Figure 4. IGF1 is a TME factor that is necessary for tumor progression.

Figure 4.

A, Expression of growth factors in tumors in comparison to normal cerebelli. B, Expression levels of IGF1 and HGF, but not PDGFA, were much higher in the tumor mass than in purified tumor GNPs (n=8). C, HGF did not promote tumor cell proliferation. D,E, IGF1 promoted cell proliferation of both mouse (D) and human tumor cells (E). F, Inactivation of IGF1R in GNPs delayed tumor progression (n≥12 for each genotype). G, Inactivation of IGF1R in GNPs prolonged mouse survival. While all IGF1R+/+ (n=7) and IGF1R−/+ (n=8) tumor mice died before P90, all IGF1R-null mice (n=14) survived till the experimental endpoint (P130). H, The medulloblastoma model in which IGF1R can be inactivated after tumor onset. I, Tamoxifen (150 mg/kg) induced inactivation of IGF1R in tumor GNPs suppressed tumor progression (n=8 for each group).

Scale bar: F and I=1mm. Data are Means±SD, Student’s t-test (C, D and E) or one-way ANOVA (F and I); n.s: not significant,**p<0.01; ***p<0.001; ****p<0.0001.

See also Figure S4