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. 2020 May 21;9:e50138. doi: 10.7554/eLife.50138

Figure 7. Schwann cells lacking YAP/TAZ fail to myelinate regenerated axons.

Ultrastructural and light microscopic analyses of remyelination in distal nerves of WT or Yap/Taz iDKO, 12–13 days after nerve crush. (A) Low magnification views of longitudinal sections of intact or crushed nerves of WT and iDKO, showing no myelination of regenerated axons in crushed nerves of iDKO as indicated by the lack of MBP immunostaining. Refer to Figure 5B for robustly regenerated axons in the same iDKO mouse. (B, C) High magnification views of boxed area in (A), showing abundant regenerated axons in crushed nerves of both WT (B) and iDKO (C). Note that regenerated axons in iDKO are not myelinated. Axons and myelin are marked by TuJ1 and MBP, respectively. (D) Semi-thin sections stained with toluidine blue showing numerous myelinated axons in crushed nerves of WT but not in iDKO. (E) TEM images of representative single large axons, myelinated in WT (left panel) but unmyelinated in iDKO (right panel). (F) Quantification of the percentage of single axons that are myelinated. n = 4 mice for WT and three mice for iDKO. *p=0.0323, Mann-Whitney. (G) G-ratio in WT and iDKO. Myelinated axons in WT are compared to unmyelinated single axons in iDKO. n = 3 mice per genotype. *p=0.0495 Mann-Whitney. Scale bars = 500 μm (A), 100 μm (B, C), 10 μm (D), 2 μm (E).

Figure 7—source data 1. Source files for TEM data.
This zip archive contains the TEM images for one WT and one iDKO used for quantitative analysis shown in Figure 7F and G. Images were taken using a JEOL 1010 electron microscope fitted with a Hamamatsu digital camera and AMT Advantage image capture software. Contrast of the images was adjusted using Photoshop software. The images in this archive were also used for the analysis in Figure 5.
Figure 7—source data 2. Source files for graphs quantifying TEM data.
This zip archive contains the raw data for WT and iDKO used for the quantitative analysis shown in Figure 7F and G. The data are contained in both a text document and an Excel file, both labeled as Mann Whitney data. These files also contain data for Figure 3—figure supplement 1, Figures 3, 4, 5, 6, 8A, Figure 8—figure supplement 1E.

Figure 7.

Figure 7—figure supplement 1. Additional images of axon regeneration and remyelination in WT and Yap/Taz iDKO.

Figure 7—figure supplement 1.

High magnification views of longitudinal sections of intact or crushed nerves of WT and iDKO, 12–13 days after nerve crush. Axons and myelin are marked by TuJ1 (green) and MBP (red), respectively. Numerous axons regenerated in crushed nerves of iDKO, as in WT, but they are unmyelinated. Myelin remains ample, as indicated by abundant MBP, in contralateral intact nerves of iDKO at 12–13 dpi. Scale bar = 50 μm.