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. 2020 May 21;9:e50138. doi: 10.7554/eLife.50138

Figure 9. Redifferentiation of Schwann cells lacking YAP/TAZ.

Figure 9.

Longitudinal sections of crushed nerves of WT and Yap/Taz iDKO at 12 dpi, immunostained by various markers of SC dedifferentiation (c-Jun and Oct-6), proliferation (Ki67) and redifferentiation (Krox20). SCs are marked by Sox10. (A) Representative sections showing c-Jun+ SCs markedly reduced in iDKO, as in WT. (B) Representative sections showing rarely observed Ki67+ proliferating SCs in iDKO, as in WT. (C) Representative sections showing Oct-6+ SCs reduced in iDKO, as in WT. (D) Representative sections showing failed upregulation of Krox20 in iDKO SCs. (E) Quantitative comparison of c-Jun+ SCs at 5 and 12 dpi, showing similar downregulation of c-Jun in WT and iDKO SC. n = 3 mice per genotype, 2-way ANOVA, ns = not significant. WT five dpi vs WT 12 dpi, **p=0.0069; WT five dpi vs iDKO five dpi, p=0.4260; WT 12 dpi vs iDKO 12 dpi, p=0.9574; iDKO five dpi vs iDKO 12 dpi, **p=0.0018. (F) Quantitative comparison of Ki67+ SCs, showing similar reduction in proliferating SCs in WT and iDKO nerves between 5 dpi and 12 dpi. n = 3 mice per genotype, 2-way ANOVA, ns = not significant. WT five dpi vs WT 12 dpi, ****p<0.0001; WT five dpi vs iDKO five dpi, p>0.9999; WT 12 dpi vs iDKO 12 dpi, p=0.6775; iDKO five dpi vs iDKO 12 dpi, ****p<0.0001. (G) Quantitative comparison of Oct-6+ SCs, showing significant downregulation of Oct-6 in WT and iDKO SCs between 5 dpi and 12 dpi. n = 3 mice per genotype, ns = not significant, 2-way ANOVA. WT five dpi vs WT 12 dpi, ***p=0.0005; WT five dpi vs iDKO five dpi, p=0.9817; WT 12 dpi vs iDKO 12 dpi, *p=0.0221; iDKO five dpi vs iDKO 12 dpi, *p=0.0299. (H) Quantitative comparison of Krox20+ SCs, showing upregulation of Krox20 in WT SCs, but not in iDKO SCs between 5 dpi and 12 dpi. n = 3 mice per genotype, 2-way ANOVA, ns = not significant. WT five dpi vs WT 12 dpi, ****p<0.0001; WT five dpi vs iDKO five dpi, p>0.9999; WT 12 dpi vs iDKO 12 dpi, ****p<0.0001; iDKO five dpi vs iDKO 12 dpi, p>0.9999. Scale bar = 10 μm (A–D).

Figure 9—source data 1. Source files for Krox20+ SC data.
This zip archive contains the IHC for one WT and one iDKO used for quantitative analysis shown in Figure 9E. Leica SP8 confocal lif images were processed using Imaris software and saved as tiffs.
Figure 9—source data 2. Source files for graphs quantifying c-Jun+ SCs, Ki67+ SCs, Oct6+ SCs and Krox20+ SCs.
This zip archive contains the raw data for WT and iDKO used for the quantitative analysis shown in Figure 9E, F, G and H. The data are contained in GraphPad Prism files, as indicated.