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. 2020 May 15;23(6):101166. doi: 10.1016/j.isci.2020.101166

Figure 2.

Figure 2

Representative Immunohistochemical Results of Different Segments from EUS-α-Syn PFFs and PBS TgM83+/− Mice at 6 mpi

(A–R) Pathological α-Syn was stained with anti-phospho-α-Syn (Ser 129) antibody for EUS-α-Syn PFFs TgM83+/− mice (A–I) and EUS-PBS TgM83+/− mice (J–R). Representative images displayed the distribution of pα-Syn in EUS (A1, J), pelvic ganglia (B1, K), S1 (C1, L), L6 (D1, M), L2 (E1, N), T2 (F1, O), cerebellum (G1, P), pons (H1, Q), and midbrain (I1, R). (A2–I2, C3–I3, H4) Higher-magnification views relative to the main image.

(S) Quantification of pα-Syn immunoreactivity of different segments from EUS-α-Syn PFFs and EUS-PBS TgM83+/− mice at 6 mpi. EUS-α-Syn PFFs TgM83+/− mice, n = 20; EUS-PBS TgM83+/− mice, n = 10.

Data are the means ± SD. Statistics were analyzed employing the Student's t test and Mann-Whitney test. ∗p < 0.05 indicates a significant difference between α-Syn PFFs groups and PBS groups. Scale bars, 500 μm in (A1, G1, H2, P); 250 μm in (C1–F1, L–O); 100 μm in (A2, B1); 50 μm in (B2, G2, I2, G3–I3, H4); 40 μm in (J, K); 25 μm in (C2–F2, C3–F3); 1 mm (H1, I1, Q, R).] DLPAG, dorsolateral periaqueductal gray; DMPAG, dorsomedial periaqueductal gray; DpG, deep gray layer of the superior colliculus; DpMe, deep mesencephalic nucleus; DpWh, deep white layer of the superior colliculus; EW, Edinger-Westphal nucleus; GiA, gigantocellular reticular nucleus; IntA, interposed cerebellar nucleus, anterior part; IPC, interpeduncular nucleus, caudal subnucleus; IPL, internal plexiform layer of the olfactory bulb; IPR, interpeduncular nucleus, rostral subnucleus; IRt, intermediate reticular nucleus; Lat, lateral (dentate) cerebellar nucleus; LDTg, tegmental nucleus; LPAG, lateral periaqueductal gray; LVe, lateral vestibular nucleus; Med, medial (fastigial) cerebellar nucleus; MVePC, medial vestibular nucleus; parvocellular part; MVe, medial vestibular nucleus; PnC, pontine reticular nucleus, caudal part; Pr5DM, principal sensory trigeminal nucleus, dorsomedial part; Pr5VL, principal sensory trigeminal nucleus, ventrolateral part; RLi, rostral linear nucleus of the raphe; RMg, raphe magnus nucleus; SuVe, superior vestibular nucleus.