CORRIGENDUM FOR “Inhibition of Human Adrenocortical Cancer Cell Growth by Temozolomide in Vitro and the Role of theMGMT Gene”

In the above-named article by Creemers SG, van Koetsveld PM, van den Dungen ESR, Korpershoek E, van Kemenade FJ, Franssen GJH, de Herder WW, Feelders RA, and Hofland LJ (J Clin Endocrinol Metab. 2016;101(12):4574–4584; doi: 10.1210/jc.2016-2768), an error appears in the text and in the figures.

The temozolomide concentration is tenfold higher than indicated, and thus the concentration reported in the text should be multiplied by a factor of ten. Consequently, the following three sentences should be rephrased in the Discussion section of the article:

(page 4580) “Importantly, the antitumor effects of TMZ shown in this study were present at concentrations of TMZ that can be achieved in the plasma of patients with advanced cancers (∼25–55 μM) (28–30).”

should read

“Concentrations of TMZ that can be achieved in the plasma of patients with advanced cancers is approximately 25–55 μM) (28–30).”

(page 4582) “Comparison of the IC₅₀ values of TMZ on ACC cell lines and glioma cell lines shows that the IC₅₀ values of TMZ in the ACC cell lines are comparable with the highly sensitive glioma cell lines (IC₅₀ 38–44 μM vs 22.5–52.4 μM). These glioma cell lines are MGMT hypermethylated (33). In contrast, ACC cell lines appear to have a low MGMT promoter methylation, which may also explain why treatment with the demethylating drug AZA minimally effected MGMT mRNA expression.”

should read

“Comparison of the IC₅₀ values of TMZ on ACC cell lines and glioma cell lines shows that the IC₅₀ values of TMZ in the ACC cell lines are comparable with the least sensitive glioma cell lines (IC₅₀ 380–440 μM vs 371–441.6 μM). These glioma cell lines also have low MGMT methylation (33).”

(page 4583) “In conclusion, we hypothesize that, based on the low MGMT methylation and meanwhile the responsiveness to TMZ, efficacy of TMZ in ACC cells does not seem to be limited by low methylation of MGMT. This is in contrast to glioblastomas.”

should read

“In conclusion, we hypothesize that, based on the low MGMT methylation and meanwhile the responsiveness to TMZ comparable with unresponsive glioma cell lines, efficacy of TMZ in ACC cells might be limited by low methylation of MGMT.”

Figures with the correct concentrations are shown here, and a newsupplemental Figure 1 is now available.

Figure 1.

Figure 2.

Figure 3.

Figure 4.