Figure 7.
Inhibition of calpain attenuates capsaicin-induced ablation of TRPV1-LN afferent terminals in hindpaw. A, Longitudinal in vivo two-photon imaging of GFP+ afferent terminals in intact hindpaw of a mouse expressing mGFP from the locus of TRPV1 (TRPV1mGFP). To assess changes of GFP+ afferent terminals, the same site was imaged repeatedly before (baseline) and 3 d after intraplantar injection of vehicle or MDL28170 (10 μm in 10 µl) followed by capsaicin (10 µg). The images represent projected image stacks across 100–150 µm (200–300 z-axis sections at 0.5 µm). Scale bar: 50 µm. B, In each stacked image, the proportion of GFP+ pixels/total pixels in the imaged area was calculated. N = 7 hindpaws from 5 mice per group; **p < 0.01 in Sidak post hoc assay following two-way ANOVA.