Figure 5.

Inducing the formation of multicompartment Golgi alters microtubule polarity in the axons of Khc mutant neurons. (A and B) Axons of Khc^E177K/27 mutant neurons contain fewer multicompartment Golgi outposts than nudE^39A/Df mutants, despite have similar or more numbers of ManII- and GalNacT2-positive compartments. Overexpressing GM130 increases the percentage of multicompartment outposts in Khc^E177K/27 mutant axons. *P = 0.05–0.01, **P = 0.01–0.001 and ****P < 0.0001; Kruskal–Wallis test with post hoc Dunn’s multiple comparison analysis (% multicompartment Golgi outposts) and one-way ANOVA with Tukey’s post hoc analysis (# outposts). Scale bar, 10 µm. Closed arrowheads indicate multicompartment outposts and open arrowheads indicate single compartments. (C) In contrast to nudE^39A/Df mutants, Khc^E177K/27 mutant axons have normal microtubule polarity. *P = 0.05–0.01, ***P = 0.001–0.0001, and ****P < 0.0001; Kruskal–Wallis test with post hoc Dunn’s multiple comparison analysis (% comets); Fisher’s Exact test (% axons). (D) The increase in multicompartment Golgi outposts in Khc^E177K/27 mutant axons that results from the overexpression of GM130 is accompanied by an increase in misoriented axonal microtubules. **P = 0.01–0.001; Kruskal–Wallis test with post hoc Dunn’s multiple comparison analysis. All data are mean ± SD. n.s., not significant; RFP, red fluorescent protein.