Skip to main content
. 2020 May 5;39(23):4551–4566. doi: 10.1038/s41388-020-1312-6

Fig. 4. LRRC4 restores GBM cell sensitivity to TMZ.

Fig. 4

a, b U251 cells (left) and PG2 cells (right) that stably expressed control vector or LRRC4 (C4) were treated with different concentrations of TMZ. Cell viability was evaluated by CCK-8. c, d Apoptosis was detected by flow cytometry in U251 cells (left) and PG2 cells (right) that stably expressed control vector or LRRC4 (C4). The cells were treated with CQ (chloroquine) and TMZ (temozolomide). The images are representative of three independent experiments. e, f Statistical analysis was performed to calculate the apoptosis rate in c and d. g, h Cleaved caspase 3, 7 and parp expression was detected by western blotting in U251 cells (left) and PG2 cells (right). The cells were treated with CQ and TMZ. Vector: control plasmid, C4: LRRC4 plasmid.