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. 2020 Jun 4;9:e48730. doi: 10.7554/eLife.48730

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional information
Genetic reagent (D. melanogaster) y[1]w[*] Pfeiffer et al., 2008
Genetic reagent (D. melanogaster) UAS-shibirets1 Pfeiffer et al., 2012 FLYB: FBst0066600; RRID:BDSC_66600
Genetic reagent (D. melanogaster) UAS-GCaMP6m FLYB: FBst0042750; RRID:BDSC_42750 FlyBase symbol: Dmel\PBac{20XUAS-IVS-GCaMP6m}VK00005
Genetic reagent (D. melanogaster) UAS-Dop2R-RNAi Dietzl et al., 2007 FLYB: FBsf0000079969;
VDRC ID: 11471
FlyBase symbol: Dmel\dsRNA-GD11471
(currently unavailable)
Genetic reagent (D. melanogaster) UAS-Dop1R1-RNAi Keleman et al., 2009) FLYB: FBsf0000090794; dsRNA-KK107258
VDRC ID: 100249
FlyBase symbol: Dmel\dsRNA-KK107258
Genetic reagent (D. melanogaster) UAS-Dop1R2-RNAi Dietzl et al., 2007 FLYB: FBsf0000073893
dsRNA-GD3391
VDRC ID: 3391
FlyBase symbol: Dmel\dsRNA-GD3391
(currently unavailable)
Genetic reagent (D. melanogaster) trans-Tango: UAS-myrGFP, QUAS-mtdTomato (3xHA); brp-SNAP Kohl et al., 2014, Talay et al., 2017
Genetic reagent (D. melanogaster) R58E02-Gal4 Liu et al., 2012 FLYB: FBtp0061564; RRID:BDSC_41347 Flybase Symbol: P{GMR58E02-GAL4}
Genetic reagent (D. melanogaster) R58E02; mcD8::GFP this paper FLYB: FBtp0061564;
RRID:BDSC_41347
FLYB: FBti0200979;RRID:BDSC_79626,
Flybase Symbol: P{GMR58E02-GAL4}
Flybase Symbol: Dmel\P{10XUAS-mCD8GFP-APEX2}attP40
Genetic reagent (D. melanogaster) HL9-Gal4 Claridge-Chang et al., 2009 FLYB: FBtp0073020
RRID:BDSC_69208
Flybase Symbol: P{HL9-GAL4.DBD}attP2
Genetic reagent (D. melanogaster) MB058B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135107
Genetic reagent (D. melanogaster) MB109B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135157
Genetic reagent (D. melanogaster) MB040B-split-Gal4 Aso et al., 2014a N/A
Genetic reagent (D. melanogaster) MB042B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135092
Genetic reagent (D. melanogaster) MB188B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135236
Genetic reagent (D. melanogaster) MB032B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135083
Genetic reagent (D. melanogaster) MB087C-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135135
Genetic reagent (D. melanogaster) MB301B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135349
Genetic reagent (D. melanogaster) MB002B-split-Gal4 Aso et al., 2014a FlyLight Robot ID:2135053
Genetic reagent (D. melanogaster) MB011B- split-Gal4 Aso et al., 2014a FlyLight Robot ID:2135062
Genetic reagent (D. melanogaster) MB-210B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135258
Genetic reagent (D. melanogaster) MB018B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135069
Genetic reagent (D. melanogaster) MB399B-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2501738
Genetic reagent (D. melanogaster) MB074C-split-Gal4 Aso et al., 2014a FlyLight Robot ID: 2135112
Antibody α-GFP (Rabbit polyclonal) Life Tech Cat#A11122 (1:1000)
Antibody α-HA (Rat monoclonal) Roche Cat#11867423001 (1:100)
Antibody Goat α-Rabbit AF488 Life Tech Cat#A11034 (1:400)
Antibody Goat α-Rat AF568 Life Tech Cat#A11077 (1:400)
Antibody α-dopamine (Mouse monoclonal) Millipore Cat#MAB5300 (1:40)
Antibody Goat α-mouse AF488 Thermo Cat#A11029 (1:200)
Sequence-based reagent CG13646F Petruccelli et al., 2018 PCR primers AGTTTGACATCCACCCCGTC
Sequence-based reagent CG13646R Petruccelli et al., 2018 PCR primers CTCACTGGCGATTCCGATGA
Sequence-based reagent Dop2RF Petruccelli et al., 2018 PCR primers CTGAACTGCACCAACGAGACGC
Sequence-based reagent Dop2RR Petruccelli et al., 2018 PCR primers CAGGATGTTGCCGAAGAGGGTC
Sequence-based reagent Dop1R1F this paper PCR primers CCGTCGTGTCCAGCTGTATCAG
Sequence-based reagent Dop1R1R this paper PCR primers CTTCTCGGCCACCTCACCTG
Sequence-based reagent Dop1R2F this paper PCR primers CCTGGCTCGGCTGGATCAAC
Sequence-based reagent Dop1R2R this paper PCR primers ATCGTGGGCTGGTACTTGCG