Skip to main content
. 2020 May 26;147(10):dev183996. doi: 10.1242/dev.183996

Fig. 6.

Fig. 6.

The effects of Hhip1 and Hhip:CD4 on the NT are a direct consequence of Shh depletion. (A-I) In situ hybridization for Gli3, Gli1 and Hhip1 following electroporation of the depicted plasmids. (J-O) In situ hybridization for Ptc2 following late or early electroporations of control GFP, Hhip1 or Hhip:CD4. Asterisks mark the electroporated sites. Control GFP had no effect on the bilateral expression of any of the genes in the NT. In contrast, Hhip1 and Hhip:CD4 transfected to the sclerotome reduced Gli1, Hhip1 and Ptc2 mRNAs unilaterally (D-O, arrowheads) but not Gli3 mRNA (A-C). (P) Quantification of the relative expression of Ptc2 in treated/contralateral sides of late and early electroporations (n=5 embryos/treatment, **P<0.01, ***P<0.001). NT, neural tube; No, notochord; Scl, sclerotome. Scale bar: 50 µm.