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. 2020 Jun 6;104(15):6693–6705. doi: 10.1007/s00253-020-10685-x

Therapeutic potential of a designed CSαβ peptide ID13 in Staphylococcus aureus-induced endometritis of mice

Bing Li 1,2,#, Na Yang 1,2,#, Yuxue Shan 1,2,3,#, Xiumin Wang 1,2, Ya Hao 1,2, Ruoyu Mao 1,2,✉, Da Teng 1,2,✉, Huan Fan 3, Jianhua Wang 1,2,✉
PMCID: PMC7275135  PMID: 32506158

Abstract

Staphylococcus aureus is a common pathogen that can cause clinical and subclinical endometritis in humans and animals. In this study, a designed CSαβ peptide ID13 from DLP4 exhibited high stable antibacterial activity in simulated gastric fluid (90.79%), serum (99.54%), and different pH buffers (> 99%) against S. aureus CVCC 546 and lower cytotoxicity (89.62% viability) than its parent peptide DLP4 (74.14% viability) toward mouse endometrial epithelial cells (MEECs). ID13 caused a depolarization of bacterial membrane and downregulation of the expression of genes involved in membrane potential maintenance and biofilm formation. The in vitro efficacy analysis of ID13 showed a synergistic effect with vancomycin, ampicillin, rifampin, and ciprofloxacin; intracellular antimicrobial activity against S. aureus CVCC 546 in MEECs; and the ability to inhibit lipoteichoic acid-induced pro-inflammatory cytokines from RAW 264.7. In the S. aureus-induced endometritis of mice, similar to vancomycin, ID13 remarkably alleviated pathological conditions, inhibited the production of cytokines (TNF-α, IL-1ß, IL-6, and IL-10), and suppressed the TLR2-NF-κB signal pathway. Collectively, these results suggest that ID13 could be a potential candidate peptide for therapeutic application in S. aureus-induced endometritis.

Key Points

•Higher antibacterial activity and lower hemolysis of ID13 than DLP4.

•ID13 could downregulate the genes of bacterial survival and infection.

•ID13 could alleviate the S. aureus-induced endometritis of mice.

•ID13 could regulate the cytokines and suppress the TLR2-NF-κB signal pathway.

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Electronic supplementary material

The online version of this article (10.1007/s00253-020-10685-x) contains supplementary material, which is available to authorized users.

Keywords: Therapeutic potential, CSαβ peptide, Staphylococcus aureus, Transcriptome sequencing, Endometritis

Introduction

Staphylococcus aureus is a facultative pathogen that can cause myriad infectious diseases in humans and animals (Fluit 2012; Foster 2012; Lai et al. 2018; Tong et al. 2015), including endometritis, a common reproductive disease (Sheldon and Owens 2017), and severely impaired reproductive performance (Gilbert et al. 2005); if not controlled, it may promote the development of septicemia and sepsis (Skovbakke and Franzyk 2017). It is estimated that 340 million women get bacterial infections in the uteri each year, and 15~20% animals develop clinical or subclinical endometritis beyond 3 weeks post-partum, costing billion dollars for treatments annually (Turner et al. 2012). In this study, our focus is mainly on breeding animals, such as cows and sows, which are more susceptible to pathogenic infections under intensive cultivation pressure. Antibiotics are widely used in the clinical prevention and treatment of endometritis, which has increased the emergence of antibiotic-resistant bacteria, especially multi-drug resistant (MDR) bacteria (Eslami et al. 2015). It has been found that S. aureus is resistant to tetracycline (43.5%), penicillin (81%), erythromycin (44.5%), clindamycin (51.2%), and ciprofloxacin (30%) (Wu et al. 2019). Meanwhile, the traditional screening of new antibacterial has suffered a considerable decline (da Cunha et al. 2017). This not only affects the treatment options but also may endanger public health (Coyne et al. 2019; Coyne et al. 2016). In recent years, antimicrobial peptides (AMPs) have attached attention of scientists by their properties such as broad-spectrum antimicrobial activity and non- or low resistance of bacteria.

Among AMPs, insect defensins (with 32–52 residues) are a large group of evolutionarily conserved cationic, cysteine-rich peptides and display a broad-spectrum activity against bacteria, fungi, and virus. They share a cysteine-stabilized αβ motif (CSαβ), which has been proved to be a valuable structural template for the development of novel antimicrobials (Koehbach 2017). However, natural insect defensins often have low activity and some toxicity toward mammalian cells, which limit their therapeutic application (Barreto-Santamaria et al. 2019). Therefore, some engineered peptides were designed based on the CSαβ scaffold, such as tenecin 1, Def-AcAA, and NZ2114, and they exhibited improved antibacterial activity and reduced cytotoxicity (Ahn et al. 2006; Landon et al. 2008; Zhang et al. 2014). Although, many attempts have been made on the medical application of AMPs, few of them have been introduced into the market yet (Kang et al. 2017; Yi et al. 2014). The screening and testing of effective AMPs (or designer AMPs) for therapeutic applications are ongoing in the pharmaceutical industry (Andersson et al. 2016).

In our previous study, a designed CSαβ peptide ID13 from DLP4 showed enhanced activity (MIC 0.95~1.91 μM), reduced hemolysis, and cytotoxicity toward mouse macrophages RAW 264.7 (Li et al. 2020). In the present study, effects of ID13 on bacterial membrane and gene expression of S. aureus CVCC 546 were explored, and its in vitro and in vivo therapeutic efficacies were evaluated through AMP-antibiotic synergism, intracellular antimicrobial action, lipoteichoic acid (LTA) neutralization, and mouse endometritis model.

Materials and methods

Strains, cell lines, and reagents

The bacterial strains Staphylococcus epidermidis ATCC 12228 and Escherichia coli ATCC 25922 were purchased from American Type Culture Collection (ATCC). S. aureus CVCC 546, Streptococcus pneumoniae CVCC 2350, Streptococcus suis CVCC 3928, Salmonella pullorum CVCC 533, and Salmonella Enteritidis CVCC 3377 were purchased from the China Veterinary Culture Collection Center (CVCC). Mouse macrophages RAW 264.7 and endometrial epithelial cells (MEECs) were obtained from Peking Union Medical College and iCell Bioscience Inc. (Shanghai, China), respectively. LTA and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (China). Antibiotics used in the study were purchased from Meilun Biotech Co., Ltd (Dalian, China). Peptides ID13 and DLP4 were expressed and purified in our lab as previously described (Li et al. 2020), with the purity of 91.2% and 92%, respectively. Other reagents were of analytical grade.

Bioavailability of peptides

Antimicrobial activity

The antimicrobial activity of peptides was calculated by minimal inhibitory concentration (MIC) via microtiter plate assay as previously depicted (Wiegand et al. 2008). Each test was conducted in triplicate.

Peptide stability in biological fluids

The stability of peptide ID13 in simulated gastric fluid (SGF), simulated intestinal fluid (SIF), and mouse serum was carried out as previously described (Benincasa et al. 2010; Liu et al. 2013; Yu et al. 2019). Simply, a final concentration of 100-μg/mL ID13 was prepared with SGF, SIF, or 25% serum and incubated at 37 °C. At different time intervals, an aliquot of 30-μL ID13 mix was taken and tested against S. aureus CVCC 546 by drop diffusion assay (Cerovsky et al. 2011). ID13 (100 μg/mL) prepared with PBS was used as a positive control; SGF, SIF, and 25% serum were used as negative controls. The agar plates were incubated overnight at 37 °C, and inhibition zones were determined.

Anti-S. aureus activity of ID13 at different pH

The antimicrobial activity of ID13 (100 μg/mL) in different pH buffers (pH 2.0, 4.0, 6.0, 8.0, and 10.0) was tested (Yu et al. 2019). After incubated with different pH buffers at 37 °C for 3 h, 30-μL ID13 and corresponding controls were dropped on Mueller-Hinton agar (MHA) plate and incubated overnight at 37 °C for anti-S. aureus activity determination.

Mammalian cytotoxicity

The effect of peptides on MEECs’ viability was evaluated by using the MTT assay as depicted before (Wang et al. 2018).

Effects of ID13 on S. aureus membrane and gene expression

Membrane depolarization

Membrane depolarization was carried out as depicted previously with some slight modifications (Kindmark et al. 2001; Yang et al. 2019). S. aureus CVCC 546 cells at 108 CFU/mL were treated with peptides at 1 ×, 2 ×, or 4 ×MIC. After incubation at 37 °C for 2 h, the bacteria were washed twice with PBS buffer and resuspended. A final concentration of 1-μM Rhodamine 123 (Rh123) was added. The cells without peptide treatment were set as negative control. After incubation at 37 °C for 30 min, Rh123 fluorescence from the cytoplasm was detected by flow cytometry.

Transcriptome sequencing

S. aureus CVCC 546 at 2.5 × 108 CFU/mL was inoculated into 250-mL Erlenmeyer flasks. The cultures were supplemented with peptide ID13 at subinhibitory concentration (4 μg/mL) at 37 °C for 60 min. RNA extraction was then performed using an RNeasy mini kit (Qiagen). The RNAs from three biological replicates were pooled; library preparation and RNA sequencing (RNA-seq) were conducted in Novogene (Beijing, China). Bioinformatic analysis was carried out using the CLC Genomics Workbench. Primary sequencing reads were mapped to the reference transcriptome and genome of S. aureus TW20 (GenBank accession number FN433596). The abundances of transcripts were generated in fragments per kilobase of transcript sequence per million base pairs sequenced (FPKM) (Trapnell et al. 2010). Differential expression analysis was assessed with an R package, where |log2 (fold change)| > 1 and q value < 0.005 were used as screening criteria for differential genes (Wang et al. 2010). For function and pathway analysis, we used the GOseq software (Young et al. 2010) and KEGG database (http://www.genome.jp/kegg/). The raw sequence data are publicly accessible at https://bigd.big.ac.cn/gsa/browse/CRA002331.

Efficacies of peptide ID13 in vitro

ID13-antibiotic synergism

Combinatory interactions of ID13 and antibiotics were tested by using the checkerboard titration method as described previously (Zhang et al. 2014).

Intracellular antimicrobial activity

S. aureus can internalize into host cells to avoid being killed by traditional antibiotics (Wang et al. 2019). To explore the intracellular antimicrobial activity of ID13, MEECs prepared in DMEM free of antibiotics were dispensed in 12-well plates at 2.5 × 105 cells/mL and 750 μL/well. After 24-h incubation, an equal volume of S. aureus CVCC 546 at 2.5 × 107 CFU/mL was supplemented and coincubated with MEECs for 0.5 h. Then, 50 μg/mL of lysostaphin was added and incubated for 1 h to kill extracellular bacteria. After washing with PBS, the MEECs were treated with different concentrations of peptides or vancomycin for 24 h and processed for colony counting (Di Grazia et al. 2014).

LTA neutralization

The anti-inflammatory activity of peptide ID13 was analyzed as depicted previously with some modifications (Liu et al. 2013; Skovbakke and Franzyk 2017). The mouse macrophages RAW 264.7 were resuspended in DMEM to a density of 1 × 106 cells/mL; an aliquot of 500 μL/well was seeded in 24-well plates for 24-h incubation. A final concentration of 10-μg/mL LTA was then added and incubated for another 3 h. After washing with PBS, cells were treated with peptides at different concentrations. Cells treated with DLP4 and vancomycin or ones untreated were used as positive and negative controls. The supernatants were harvested and analyzed for cytokine levels of TNF-α, IL-1ß, IL-6, and IL-10 using an enzyme-linked immunosorbent assay (ELISA) kit (R&D systems, USA). Each group was made in triplicate.

Efficacies of peptide ID13 in mice endometritis model

The animal procedures were approved by the Institutional Animal Care and Use Committee of Chinese Academy of Agricultural Sciences (CAAS). Animal studies were performed with female CD-1 mice, weight 25 g. Sixty mice were randomly divided into six groups (n = 10). To induce endometritis, 100-μL S. aureus CVCC 546 (1 × 108 CFU/mL) was injected into the uteri of mice via vagina for 3 days with a 1-mL syringe. Twenty-four hours post-infection, mice were administered intraperitoneally (i.p.) with peptides at 5, 10, or 15 mg/kg once daily for 3 days. Mice raised without any treatment, treated with vancomycin, or treated with PBS were used as the blank, positive, and negative controls, respectively. At 24 h post-treatment, peripheral blood and the uterine tissues were collected from the mice. Peripheral blood (n = 5) from the negative and blank control was used for white blood cell analysis; the others (n = 5) from each group were centrifuged; and the serum was used for TNF-α, IL-1ß, IL-6, and IL-10 analysis by using the ELISA kit (R&D systems, USA). Uteri (n = 5) from each group were homogenized; the homogenate was diluted with a 1: 10 (w/v) ratio and dropped on Baird-Parke (BP) agar plate for pathogenic bacteria isolation and identification. Segments from another five uteri in each group were fixed in 4% paraformaldehyde for histopathological analyses by hematoxylin-eosin (H&E) staining; the rest ones were extracted for TLR2 and NF-κB pathway analysis by western blot (Li et al. 2015).

Statistical analysis

All data were analyzed with GraphPad Prism 7 and presented as mean ± standard deviation (SD), where “n” represents the number of animals or samples. Comparisons between groups were performed by one-way analysis of variance (ANOVA) followed by Dunnett’s multiple test. p < 0.05 was considered statistically significant.

Results

Bioavailability of peptide ID13

Antimicrobial activity

As shown in Table 1, the activity of ID13 with MICs of 0.95~1.91 μM was higher than that of its parent peptide DLP4 with MICs of 3.75~14.99 μM against S. aureus CVCC 546, S. epidermidis ATCC 12228, S. pneumoniae CVCC 2350, and S. suis CVCC 3928, respectively. However, similar to DLP4, ID13 had no activity against Gram-negative bacteria (MIC > 30.5 μM).

Table 1.

Antibacterial activity of peptides against pathogenic strains.

Peptides MICs (μM)
S. aureus CVCC 546 S. epidermidis ATCC 12228 S. pneumonia CVCC 2350 S. suis CVCC 3928 E. coli ATCC 25922 S. pullorum CVCC 533 S. Enteritidis CVCC 3377
ID13 0.95 1.91 0.95 0.95 >30.50 >30.50 >30.50
DLP4 3.75 14.99 7.50 3.75 >29.98 >29.98 >29.98
Vana 0.67 0.67 0.34 0.17 86.15 86.15 86.15
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aVancomycin

Stability of peptide ID13 in biological fluids

Proteases in the gastrointestinal tract and blood limit the use of AMPs (da Cunha et al. 2017; Yu et al. 2019). The susceptibility of peptide ID13 to pepsin, trypsin, and serum was detected by using the drop diffusion method. After treatment with SGF or 25% serum for 60 min, peptide ID13 retained 90.79% or 99.54% activity against S. aureus CVCC 546, respectively, while it has easily undergone proteolysis in less than 5 min in SIF (Fig. 1a).

Fig. 1.

Fig. 1

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Bioavailability of peptide ID13. a The stability of peptide ID13 in simulated gastric fluid (SGF), simulated intestinal fluid (SIF), serum, and b different pH buffers. NC, negative control; PC, positive control; ST, stability tested of peptide ID13 prepared in biological fluids. c Cytotoxicity of peptides ID13, DLP4, and vancomycin (Van) toward MEECs (n = 4)

The varying pH buffers (2.0 to 10.0) had no effect on ID13 activity (> 99%) against S. aureus CVCC 546 (Fig. 1b). These results indicate that ID13 can be used via oral administration.

Cytotoxicity of peptides toward MEECs

The cell viability of ID13 toward MEECs was 89.62% at a concentration of 256 μg/mL, which was nearly equal with that of vancomycin (90.15%), and showed lower cytotoxicity than that of DLP4 (74.14%) at 256 μg/mL, indicating a low cytotoxicity (Fig. 1c).

Effects of peptide ID13 on S. aureus CVCC 546

ID13-depolarized S. aureus CVCC 546 membrane

As shown in Fig. 2a, when S. aureus CVCC 546 cells were treated with peptide ID13 at 1 ×, 2 ×, or 4 × MIC for 2 h, the fluorescence intensity of Rh 123 was reduced by 23.54%, 26.49%, and 23.05%, respectively. There was no significant concentration relationship of ID13. Comparably, the fluorescence intensity from cells treated with 1 ×, 2 ×, or 4 × MIC vancomycin was dose-dependently increased by 23.1%, 26.6%, and 32.3%, respectively. The membrane depolarization by ID13 correlates with the findings of the transcriptional analyses as follows.

Fig. 2.

Fig. 2

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The effects of peptide ID13 on S. aureus. a The membrane depolarization, b differential gene expression, c enriched GO terms, and d KEGG pathway of ID13-treated S. aureus CVCC 546

ID13 regulated S. aureus CVCC 546 gene expression

To investigate the effects of ID13 on gene expression of S. aureus, transcriptome sequencing was performed. A total of 640 differentially expressed genes (DEGs) were identified, including 266 upregulated genes and 374 downregulated genes (Fig. 2b). Significant changes in response to ID13 are involved in the genes that are essential to metabolic processes and pathways such as membrane function, biosynthesis of primary and secondary metabolites, and microbial metabolism in diverse environments (Fig. 2c–d), which play an important role in cell viability and pathogenicity.

Membrane potential (ΔΨ)-related genes

ΔΨ is crucial to energy metabolism and is maintained by the transmembrane gradients of ions (Na+, K+, Cl−, and H+, etc.), which reflects the state of the bacterial physiology. A decline of ΔΨ will result in the inhibition of energy metabolism (Shapiro 2000; van der Stel et al. 2017).

As shown in Supplementary Table S1, the genes (mnhABCDEHF, fabD, lytM, ureF, and ipdC) encoding ion binding and transport proteins were reduced by 1.1- and 3.2-fold, while the other ones (ebh, mtlA, sdrC, sdrD, sdrE, copA, hutG, and zinT) were upregulated. The decreased expression of mnhABCDEHF, specifically responsible for Na+/H+ antiporter, suggested a decline of ΔΨ and energy biosynthesis (Castro et al. 2016). The genes (nreB, ftnA, narGHT, nasD, pflAB, adh1, ldhAB, and natA) for energy metabolism were upregulated (1.3 to 7.8-fold), while trxAB (encoding thioredoxin and thioredoxin reductase) (Villanueva et al. 2016), glpD (encoding glycerol-3-phosphate dehydrogenase) (Szalus-Jordanow et al. 2018), fda (encoding fructose-bisphosphate aldolase class 1) (Capodagli et al. 2014), pgk (encoding phosphoglycerate kinase) (Liew et al. 2015), and eno (encoding enolase) (Dai et al. 2019) were downregulated. The results indicated that ID13 might negatively affect the endergonic processes such as ATP synthesis through mnhABCDEHF inhibition.

Pathogenicity-related genes

S. aureus causes host infections through surface colonization, entry, and invasion process (Bayer et al. 1996). Colonization is mediated by the microbial surface component recognizing adhesive matrix molecules (MSCRAMMs), mainly encoded by clfB, fnbA, eno, ebh, spa, agrABC, and sdrCDE (Speziale et al. 2009), which are under control of sar and agr, and agr is also partly regulated by sar (Azara et al. 2017; Speziale et al. 2009). The extracellular DNA is important in the course of biofilm formation, and it is regulated by cid and lrg operon, which induces and inhibits cell lysis, respectively (Rice et al. 2007; Sadykov and Bayles 2012; Shen et al. 2015). Meanwhile, LytSR system regulates the expression of lrg; disruption of the lytSR genes induces increased autolysis (Sadykov and Bayles 2012). In addition, the genes secA (encoding a key component of the general bacterial secretion system required for toxins secretion), hly (encoding hemolysin), and cap (encoding capsular polysaccharide) also play various roles in bacterial evasion of the host immune defense (Jin et al. 2015; Weidenmaier and Lee 2017; Zhang and Austin 2005).

As shown in Supplementary Table S2, the transcript levels of clfB, ebh, spa, agrABC, and sdrCDE but not fnbA and eno were elevated 1.8 to 6.2 fold. It showed that ID13 exhibited positive effect on the expression of most MSCRAMM-encoding genes. Meanwhile, the expression of positive regulatory genes of MSCRAMMs (agrABC and sarS) was also upregulated (1.8 to 3.2- fold). The transcript levels of the positive regulator of autolysis cidA remarkably decreased by 4.2 fold, while the expression of negative regulator lrgB increased by 2.5 fold. It indicated that S. aureus might protect itself from being induced autolysis by ID13. Due to the increased expression of secA for toxin secretion, hly expression was also elevated. Compared with the control, ID13-treated S. aureus significantly downregulated the levels of genes (capABCDGLMN) for biofilm formation. These results suggested that ID13 affected S. aureus biofilm formation by inhibiting capsule polysaccharide synthesis, thereby reducing bacterial pathogenicity.

Efficacies of peptide ID13 in vitro

Synergism of peptide ID13 with antibiotics

The combination of antimicrobial peptides with antibiotics is an efficacious strategy to combat antibiotic resistance of bacteria (Zharkova et al. 2019). As shown in Fig. 3a, the FICI values for ID13 and vancomycin (Van), ampicillin (Amp), rifampin (Rif), or ciprofloxacin (Cip) ranged from 0.1875 to 0.375, indicating the synergistic effect. It demonstrated that the combined application of peptide ID13 with antibiotics has a potential to be used to inhibit the growth of S. aureus CVCC 546.

Fig. 3.

Fig. 3

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In vitro efficacy of peptide ID13. a Combined application of peptide ID13 with antibiotics against S. aureus CVCC 546, vancomycin, ampicillin, rifampin, and ciprofloxacin that are represented by Van, Amp, Rif, and Cip, respectively, and b antimicrobial activity against intracellular S. aureus CVCC 546. Inhibition of LTA-induced cytokines c TNF-α, d IL-1β, e IL-6, and f IL-10 from RAW 264.7. Statistical significance of differences between experimental and LTA groups were determined using the one-way ANOVA and Dunnett’s multiple comparison. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Intracellular antimicrobial activity

As shown in Fig. 3b, ID13, DLP4, and vancomycin could enter MEEC cells and kill over 99% of S. aureus CVCC 546 in a dose-dependent manner. The killing rate of 200-μg/mL ID13 and DLP4 was up to 99.92% and 99.90%, slightly lower than that of vancomycin (99.99%). This result demonstrated that ID13 exhibited remarkable bactericidal efficiency against intracellular S. aureus.

Anti-inflammatory activity

As shown in Fig. 3c–f, LTA could stimulate the production of TNF-α, IL-1β, IL-6, and IL-10 in RAW 264.7. After treatment with ID13, DLP4, and vancomycin, the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 decreased in a concentration-dependent manner in all tests. After incubation with 50-μg/mL ID13, the levels of cytokines TNF-α (1648.57 ± 209.29 pg/mL), IL-6 (203.9 ± 8.65 pg/mL), and IL-1β (215.17 ± 19.54 pg/mL) were reduced by 68.18%, 70.61%, and 70.44%, higher than those of DLP4. In contrast, after treatment with 25-μg/mL ID13, anti-inflammatory cytokine IL-10 was increased by 7.47%, lower than that of DLP4 (45.09%) and vancomycin (81.26%). The results suggest that ID13 has the ability to suppress pro-inflammatory responses in macrophages.

Efficacy of peptide ID13 in vivo

Given its low cytotoxicity and stability in SGF, SIF, serum, and different pH buffers, the in vivo therapeutic potential of peptide ID13 was further investigated through an endometritis model of mouse.

Morphology changes in the mice uterus

By anatomy, the uterus showed normal morphology and had no adhesion with the surrounding tissues in the blank control; comparatively, in the negative control, the uterus appeared abnormal, including enlarged uterine volume; dilated and tortuous lumen; thinned uterine wall; yellow pus or clear liquid in the lumen; and swollen, congested, and dark red perimetrium surface (Supplementary Fig. S1).

Leukocytes and pathogens analysis

In the negative and blank controls, it was observed significant changes in the white blood cells (Supplementary Table S3). The number of white blood cells (14.56 ± 3.00 109 cells/L), neutrophils (9.65 ± 2.00 109 cells/L), and lymphocytes (3.25 ± 0.83 109 cells/L) in the negative control was significantly higher than those in the blank control. The percentage of neutrophils was increased remarkably by 66.24 ± 1.51%, while that of lymphocytes was decreased significantly by 22.24 ± 2.33%. The result indicated that endometritis can be established in mice by intrauterine inoculation of S. aureus CVCC 546.

To isolate and identify S. aureus CVCC 546, homogenized uterine samples diluted 1:10 (w/v) in PBS were dropped on BP agar supplemented with potassium tellurite yolk enrichment broth, and then the isolates were further conformed through 16S rRNA gene sequence analysis. As shown in Supplementary Fig. S2a, S. aureus CVCC 546 isolates were found to be the major bacterial pathogen isolated from uteri of mouse; after amplicon and molecular identification (Supplementary Fig. S2b), 16S rRNA gene sequence BLAST on NCBI website (https://blast.ncbi.nlm.nih.gov/Blast.cgi) showed that the isolated pathogen was most similar to S. aureus according to taxonomy report (Supplementary Fig. S2c).

Ameliorating pathological uteri

Histopathological analysis of uteri by H&E staining showed no histological abnormality that was found in the blank control with normal endometrium, myometrium, and perimetrium (Fig. 4a). Comparatively, structures of the uteri in the negative control were obviously destroyed; the glands of the endometrium were atrophied and even disappeared; a large number of inflammatory cells (mainly neutrophils and lymphocytes, accompanied by a small number of monocytes) were observed in the stroma of the endometrium as well as fibrous tissue hyperplasia. In addition, inflammation also caused the shedding of epithelial cells into uterine cavity (Fig. 4b). These results showed that the endometritis model of mouse was successfully established by infection of S. aureus CVCC 546.

Fig. 4.

Fig. 4

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Histopathological analysis of uteri by H&E staining (× 200). Histopathology of uteri of the a blank, b negative (PBS), c positive (vancomycin, 10 mg/kg) control, and (d–f) groups treated with 5, 10, and 15 mg/kg peptide ID13, respectively. Red arrow, gland; yellow arrow, neutrophils; cyan arrow, lymphocytes; green arrow, the shedding of epithelial cells into uterine cavity

After administration of ID13 (5, 10, or 15 mg/kg), pathological conditions were improved rapidly in a dose-dependent manner (Fig. 4d–f). Only a small amount of scattered inflammatory cells were observed in part of uterus after treatment with 10-mg/kg vancomycin or 15-mg/kg peptide ID13 (Fig. 4 c and f). The results suggested that similar to vancomycin, ID13 could ameliorate the pathological outcome of mouse uteri infected with S. aureus CVCC 546.

Attenuating inflammatory cytokines

As shown in Fig. 5, the production of inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-10 in the negative control (PBS) was remarkably increased in serum from mice infected with S. aureus CVCC 546. After treatment with ID13, the levels of inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-10 significantly decreased in a dose-dependent manner. Administration with 15-μg/mL ID13 resulted in a reduction of cytokines TNF-α (107.80 pg/mL), IL-6 (163.14 pg/mL), IL-1β (218.64 pg/mL), and IL-10 (55.48 pg/mL), which was roughly equivalent to 10 μg/mL vancomycin (TNF-α, 124.92 pg/mL; IL-6, 134.74 pg/mL; IL-1β, 198.72 pg/mL; IL-10, 48.20 pg/mL).

Fig. 5.

Fig. 5

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Effects of ID13 on inflammatory cytokines a TNF-α, b IL-1β, c IL-6, and d IL-10 in serum from mice (n = 5). Statistical significance of differences between experimental and negative control (PBS) was determined using the one-way ANOVA and Dunnett’s multiple comparison. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Regulating NF-κB signaling pathway

TLR2 is critical for the host immune cells and can recognize microbe-associated pathogenic molecules (e.g., LTA), which stimulates the production of inflammatory cytokines and results in the activation of NF-κB signaling pathway (Goldstein 2004). To explore the anti-inflammatory mechanism of peptide ID13, the expression of TLR2 and NF-κB was analyzed by western blot. As shown in Fig. 6, the relative content of TLR2 in the negative control (PBS) was significantly higher than that in the blank control (CK). ID13 could reduce the TLR2, IκB, p-IκB, p65, and p-p65 contents in a dose-dependent manner. However, the levels of p-IκB and p-p65 were lower than those of IκB and p65, indicating a negative regulation by ID13. These results suggested that ID13 inhibited the expression of TLR2 and downregulated NF-κB signaling pathway.

Fig. 6.

Fig. 6

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Effects of peptide ID13 on TLR2-NF-κB signaling (n = 5). Statistical significance of differences between experimental and negative control (PBS) was determined using the one-way ANOVA and Dunnett’s multiple comparison. *p < 0.05, **p < 0.01, ***p < 0.001

Discussion

S. aureus is a common pathogen that can cause post-partum clinical and subclinical endometritis in humans and animals (Turner et al. 2012; Zhang et al. 2017). During the long-term use of antibiotics, S. aureus has been found to develop resistance (Hiramatsu 2001; Lai et al. 2018; Pirolo et al. 2019). To combat antibiotic-resistant S. aureus, insect defensins are promising candidates for the development of novel antimicrobials (Koehbach 2017). In this study, the efficacies of a designed peptide ID13 from insect defensin DLP4 were assessed against S. aureus CVCC 546 in vitro and in vivo.

Antimicrobial activity, stability, and cytotoxicity are important factors in the preliminary screening of candidate peptides. ID13 displayed potent antimicrobial activity (MICs 0.95~1.91 μM) against Gram-positive bacteria (Table 1); high stability in SGF (90.79%), serum (99.54%), and different pH buffers (> 99%); and lower cytotoxicity (89.62% viability) toward MEECs than its parent peptide DLP4 (74.14% viability) (Fig. 1), which may be attributed to its increased hydrophobicity, decreased charge, and shorter amino acid side chain (Li et al. 2020). These results also suggested a good bioavailability for ID13.

Structure analysis showed that ID13 is an amphipathic, cationic, and CSαβ peptide (Li et al. 2020), indicating the high affinity to anionic bacterial membranes, which was exactly confirmed in membrane depolarization of ID13 (Fig. 2a). Membrane potential is maintained by a substantial proportion of the energy and electron (Kinkel et al. 2016); depolarization of membrane disrupts the energy generation and electron transfer, which is specifically reflected in the depression of Na(+)/H(+) pump activity (Supplementary Table S1) (Castro et al. 2016). The cationic antimicrobial peptide (Temporin L-NH2, ovispirin-1-NH2, and dermaseptin K4-S4 (1-16)-NH2)-treated S. aureus showed a downregulation of nar, nas, and adh1(Pietiainen et al. 2009), which were increased in ID13-treated S. aureus in our study (Supplementary Table S1). cap is responsible for capsular polysaccharide expression (Gill et al. 2005) and plays an important role in biofilms formation (Prokopovich and Perni 2009); a significant downregulation of cap expression (Supplementary Table S2) implies a reduced bacterial infection (Weidenmaier and Lee 2017).

S. aureus can internalize into host cells to form an intracellular pathogen pool, thereby avoiding being killed by traditional antibiotics and causing further damages (Al Kindi et al. 2019; Wang et al. 2019). Meanwhile, the bacterial cell wall component LTA is an important immunostimulatory virulence factor in S. aureus infections (Ginsburg 2002), can activate pro-inflammatory signaling (Rockel and Hartung 2012), and promotes the development of sepsis (Hotchkiss and Karl 2003). NZ2114, a variant of the fungus-derived defensin plectasin from Pseudoplectania nigrella, is a CSαβ peptide, which could be internalized into the cells via clathrin-mediated endocytosis and macropinocytosis, and distributed in the cytoplasm. It exhibits intracellular bacteriostatic efficacy with the reduction of S. aureus colony counting (Wang et al. 2018). ID13 shares the same structure with NZ2114 and thus may have a similar mechanism of cell internalization to kill intercellular S. aureus like NZ2114 does. In present study, almost all of the intracellular S. aureus CVCC 546 were killed by ID13 (99.92%), slightly lower than that of vancomycin (99.99%) (Fig. 3b). In recent years, many peptides such as Temporins A, NZ2114, and LL-37 have been proved to exhibit a high efficiency against intracellular S. aureus in host cells (Di Grazia et al. 2014; Noore et al. 2013; Wang et al. 2018). Moreover, ID13 could inhibit the production of inflammation cytokines in RAW 246.7 and in mice challenged with LTA or S. aureus CVCC 546 (Figs. 3c–e and 5a–c). However, the level of IL-10 decreased in mice (Fig. 5d) might attribute to the effects of different kind of immune cells induced by LTA and S. aureus CVCC 546 in vitro and in vivo, respectively. Also, ID13 could attenuate the phosphorylation of NF-κB in mice (Fig. 6), thereby downregulating the expression of downstream pro-inflammatory cytokines (Fig. 5a–c). These results suggested that ID13 can improve the pathological conditions of mouse endometritis (Fig. 4c–f).

In conclusion, peptide ID13 exhibited stable antimicrobial activity in SGF and serum against S. aureus CVCC 546 and lower cytotoxicity than its parent peptide DLP4. ID13 caused a depolarization of bacterial membrane and downregulated the expression of genes involved in membrane potential maintenance and biofilm formation. ID13 had a synergistic effect with ancient antibiotics and intracellular antimicrobial activity of S. aureus CVCC 546 in MEECs. ID13 improved pathological conditions of endometritis of mouse induced by S. aureus and inhibited the production of TNF-α, IL-1ß, IL-6, and IL-10. Furthermore, TLR2-NF-κB signaling pathway was significantly suppressed by ID13. These results suggest that ID13 could be a potential antimicrobial for clinical application.

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Author contributions

Jianhua Wang, Ruoyu Mao, Da Teng, Xiumin Wang, and Bing Li conceived and designed experiments. Bing Li carried out all the experiments. Na Yang, Yuxue Shan, and Ya Hao prepared partial materials in laboratory; Da Teng, Ruoyu Mao, and Jianhua Wang contributed in writing. Jianhua Wang contributed in funding acquisition. Ya Hao contributed to materials and reagents. Huan Fan coordinated partially support of human resources during running.

Funding information

This study was supported by the National Natural Science Foundation of China (Grants 31872393, 31772640, and 31702146), the AMP Direction of the National Innovation Program of Agricultural Science and Technology in CAAS (Grant CAAS-ASTIP-2013-FRI-02), and its Key Project of Alternatives to Antibiotics for Feed Usages (Grant CAAS-ZDXT2018008).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

The mouse experiment was performed according to the Animal Care and Use Committee of the Feed Research Institute of Chinese Academy of Agricultural Sciences (CAAS) and approved by the Laboratory Animal Ethical Committee and its Inspection of the Feed Research Institute of CAAS (AEC-CAAS-20090609).

Footnotes

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Bing Li, Na Yang and Yuxue Shan contributed equally to this work.

Contributor Information

Ruoyu Mao, Email: maoruoyu@caas.cn.

Da Teng, Email: tengda@caas.cn.

Jianhua Wang, Email: wangjianhua@caas.cn, Email: 2681298635@qq.com.

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