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. 2020 May 7;10(5):729. doi: 10.3390/biom10050729

Figure 5.

Figure 5

UAG decoding by sup70-65 in absence of PUS1 and MET22. (A) Scheme depicting the first codon position U/G error in the decoding of UAG stop codons by sup70-65. In the trp1-1 allele, a premature UAG codon abrogates translation of the trp1-1 mRNA, leading to tryptophan auxotrophy (Trp-). In the presence of sup70-65 in single or high copy numbers, UAG readthrough occurs, leading to a partial or full reversion of tryptophan auxotrophy (Trp+) associated with the trp1-1 allele. (B) Assessment of UAG readthrough by sup70-65 in single copy (s.c.) or high copy (h.c.) numbers at different temperatures and in the absence of MET22, PUS1, or both. +Trp/+Met refers to minimal medium supplemented with both methionine and tryptophan (control), -Trp/+Met medium allowed UAG readthrough to be determined, and +Trp/-Met medium was employed as a phenotypic control for the presence of the met22 mutation that causes methionine auxotrophy.