Figure 6.
The effect of the ctp gene mutation on A. baumannii viability. (A) Confocal scanning laser microscopy images of fluorescence-stained ATCC 17978, MR14, and MR14C using a live/dead BacLightTM bacterial viability kit; scale bar = 20 µm; (B) number of dead cells obtained from fluorescence microscopy using live/dead staining; (C) microtiter fluorescent method for the quantification of live and dead bacterial percentages, a linear relationship between the percentage of live cells and the green/red fluorescence ratio from the SYTO9/propidium iodide (PI) viability assay. All experiments were performed in triplicate. **** p < 0.0001.