Table 1.
Primary and Secondary antibodies used in Western blot and immunohistochemistry (IHC).
| Primary and Secondary Antibodies | Company Cat. No | Dilution Western Blot |
Dilution IHC |
|---|---|---|---|
| Anti Neuron-Specific nuclear protein (NEUronal Nuclei Neu-N) | Monoclonal antibody Merk-Millipore Cat. MAB377 |
1:1000 | 1:500 |
| Anti-Neurofilament 200kDa, clone RT-97 | Monoclonal antibody Merk-Millipore Cat. MAB5262 |
1:1000 | 1:500 |
| Anti-Glial Fibrillary Acidic Protein (GFAP) | Monoclonal antibody Merk-Millipore USA Cat. MAB3402 |
1:1000 | 1:500 |
|
Anti-Glucose transporter 1 GLUT-1 (c-Terminus)
clone 5B12.3 |
Monoclonal antibody Merk-Millipore USA Cat. MABS132 |
1:500 | 1:500 |
| Ionized calcium binding adaptor molecule 1 (Iba-1) | Thermofisher USA Cat. PA5-27436 |
---- | 1:200 |
| Anti Aquaporine-4 (AQP4) | Monoclonal antibody Merk-Millipore USA AB3594 |
1:1000 | 1:500 |
| Anti-Mouse Biotinylated | Polyclonal antibody Bethyl Lab. Cat. A90-116B |
------ | 1:200 |
| Anti-Mouse HRP Conjugated | Polyclonal antibody Bethyl Lab. Cat. A90-116P |
1:5000 | ------ |
| Anti-Rabbit Biotinylated | Polyclonal antibody Bethyl Lab. A120-101B |
------ | 1:200 |
| Anti-Rabbit HRP Conjugated | Polyclonal antibody Bethyl Lab. Cat. A120-101P |
1:5000 | ------ |
| Anti-Mouse | Alexa Fluor 488® | ------ | 1:100 |
| Anti-Rabbit | Alexa Fluor 594® | ------ | 1:100 |