Table 4.

Experimental domain of two-level CCD of HPLC assay optimization for three factors and measured responses.

Std.	Run	Factors Levels			Responses
		pH	Column Temperature (°C)	Flow Rate (mL/min)	Rt FEX	Rt TNZ	Peak Area FEX
4	1	2.50	30.00	0.80	3.87	1.70	426,304
16	2	3.50	30.00	0.90	3.75	1.68	411,926
12	3	3.00	35.00	0.90	3.62	1.60	455,576
13	4	2.50	40.00	0.90	3.55	1.58	454,224
3	5	3.50	40.00	0.90	3.65	1.61	446,531
5	6	3.00	26.95	1.00	3.62	1.62	437,479
1	7	2.16	35.00	1.00	3.91	1.66	458,515
15	8	3.00	35.00	1.00	3.75	1.69	458,882
8	9	3.00	35.00	1.00	3.76	1.70	459,250
6	10	3.00	35.00	1.00	3.74	1.70	458,552
17	11	3.00	35.00	1.00	3.74	1.70	458,735
19	12	3.00	35.00	1.00	3.74	1.69	458,816
9	13	3.00	35.00	1.00	3.74	1.69	458,794
20	14	3.00	35.00	1.00	3.74	1.69	459,213
14	15	3.84	35.00	1.00	3.98	1.91	443,894
7	16	2.50	30.00	1.10	4.00	1.77	426,620
10	17	3.50	30.00	1.10	4.07	1.87	407,547
11	18	2.50	40.00	1.10	3.76	1.66	448,713
18	19	3.00	43.41	1.10	3.74	1.66	461,233
2	20	3.50	40.00	1.20	3.87	1.81	437,156