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. 2020 May 13;8(5):729. doi: 10.3390/microorganisms8050729

Figure 4.

Figure 4

The effect of Rab GTPase downregulation on the release of infectious EBV virions. i293/2089 cells transfected with or without Rab8a, Rab10, or Rab11a siRNAs were treated with tamoxifen for 48 h to induce the EBV lytic cycle. Their supernatants were then harvested and added to Daudi− cells. Two days later, the percentage of GFP-positive Daudi− cells was analyzed by flow cytometry. (A) Representative histograms are shown. As a control, Daudi− cells were incubated without the supernatants (thin lines). (B) The average infection frequencies and standard deviations are also represented. Results reflect three independent experiments. Statistical differences were assessed using Student’s t test and are represented as ** p < 0.01. (C) Downregulation of Rab proteins was confirmed by Western blot.