Table 1.

(A) The cumulative fractional inhibitory concentration index (∑FICI) range of HSGN-94 and HSGN-189 in combination with Tunicamycin and Targocil against MRSA ATCC 33592. (B) The cumulative fractional inhibitory concentration index (∑FICI) range of HSGN-94 and HSGN-189 in combination with Tunicamycin and Targocil against MRSA USA300. (C) The cumulative fractional inhibitory concentration index (∑FICI) range of HSGN-94 and HSGN-189 in combination with Tunicamycin and Targocil against VRE ATCC 51575. Note: ∑FICI was interpreted as follows: ∑FICI of ≤0.5 is considered to demonstrate synergy (SYN). An ΣFICI of >0.5–1.25 was categorized as additive (ADD). ΣFICI of >1.25–4 was considered as indifference (IND), while ΣFICI values of >4 were categorized as antagonistic.

A	MRSA ATCC 33592
	MIC Alone		Combination MIC		∑FICI	SYN/ADD/IND	MIC Alone		Combination MIC		∑FICI	SYN/ADD/IND
Antibiotic	Antibiotic	HSGN-94	Antibiotic	HSGN-94			Antibiotic	HSGN-189	Antibiotic	HSGN-189
Targocil	16	0.5	2	0.25	0.6	ADD	32	0.5	16	0.5	1.5	IND
Tunicamycin	256	0.5	64	0.125	0.5	SYN	256	0.5	32	0.25	0.6	ADD
B	MRSA USA300
	MIC Alone		Combination MIC		∑FICI	SYN/ADD/IND	MIC Alone		Combination MIC		∑FICI	SYN/ADD/IND
Antibiotic	Antibiotic	HSGN-94	Antibiotic	HSGN-94			Antibiotic	HSGN-189	Antibiotic	HSGN-189
Targocil	>1024	2	16	2	1.0	ADD	>1024	2	16	2	1.0	ADD
Tunicamycin	32	2	2	1	0.6	ADD	64	2	4	0.5	0.3	SYN
C	VRE Faecalis ATCC 51575
	MIC Alone		Combination MIC		∑FICI	SYN/ADD/IND	MIC Alone		Combination MIC		∑FICI	SYN/ADD/IND
Antibiotic	Antibiotic	HSGN-94	Antibiotic	HSGN-94			Antibiotic	HSGN-189	Antibiotic	HSGN-189
Targocil	>1024	2	16	2	1.0	ADD	>1024	2	16	2	1.0	IND
Tunicamycin	16	2	4	0.5	0.5	SYN	16	2	0.5	1	0.5	SYN