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. 2020 May 14;9(5):1216. doi: 10.3390/cells9051216

Figure 3.

Figure 3

Figure 3

ICMT-mediated inflammatory responses are mediated by Ras. (A) The mRNA levels of inflammatory genes (COX-2, TNF-α, IL-1β, and IL-6) in RAW264.7 cells treated with 1 µg/mL of LPS in the presence or absence of siRas were determined by real-time PCR. (B,C) The mRNA levels of inflammatory genes (COX-2, TNF-α, IL-1β, and IL-6) were also determined in HEK293 cells transfected with Myc-k-Ras or Myc-k-Ras-C185A using real-time PCR. ((D), upper and lower panels) The mRNA levels of K-RAS and inflammatory genes (IL-6, TNF-α, and IL-1β) in MDA-MB-231 cells treated with siRNA to Ras (left panel) or transfected with Myc-k-Ras or Myc-k-Ras-C186A (right panel) were determined by real-time PCR. *: p < 0.05 and **: p < 0.01 compared to the normal group, LPS-treated group, or Myc-k-Ras-transfected group.